Combined genetic-pharmacologic inactivation of tightly linked ADAMTS proteases in temporally specific windows uncovers distinct roles for versican proteolysis and glypican-6 in cardiac development

• Published in: Matrix biology Vol. 131; pp. 1 - 16
• Main Authors: Mead, Timothy J., Bhutada, Sumit, Foulcer, Simon J., Peruzzi, Niccolò, Nelson, Courtney M., Seifert, Deborah E., Larkin, Jonathan, Tran-Lundmark, Karin, Filmus, Jorge, Apte, Suneel S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2024
• Subjects: ADAMTS1 Protein - genetics; ADAMTS1 Protein - metabolism; ADAMTS5 Protein - genetics; ADAMTS5 Protein - metabolism; Animals; Degradomics; Gene Expression Regulation, Developmental; Glypican; Glypicans - genetics; Glypicans - metabolism; Heart - growth & development; Heart Defects, Congenital - genetics; Heart Defects, Congenital - metabolism; Heart Defects, Congenital - pathology; Medical and Health Sciences; Medical Biotechnology; Medicin och hälsovetenskap; Medicinsk bioteknologi; Metalloprotease; Mice; Mice, Knockout; Proteoglycan; Proteolysis; Terminomics; Versican; Versicans - genetics; Versicans - metabolism; Proteoglycan; Metalloprotease; Glypican; Terminomics; Degradomics; Versican

• Combined genetic-pharmacologic ADAMTS1 and ADAMTS5 inactivation in mice.• Degradomics identification of putative ADAMTS1 and ADAMTS5 substrates.• A requirement for versican proteolysis and glypican-6 in heart development.• Novel roles for ADAMTS1, ADAMTS5 and glypican-6 in hedgehog signaling in th...