High-quality genome sequences of uncultured microbes by assembly of read clouds

• Published in: Nature biotechnology Vol. 36; no. 11; pp. 1067 - 1075
• Main Authors: Bishara, Alex, Moss, Eli L, Kolmogorov, Mikhail, Parada, Alma E, Weng, Ziming, Sidow, Arend, Dekas, Anne E, Batzoglou, Serafim, Bhatt, Ami S
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.12.2018; Nature Publishing Group
• Subjects: 45; 45/22; 45/23; 631/208/212/2142; 631/208/212/2302; 631/326/171; 631/326/2565; 631/326/41; Agriculture; Analysis; Assembly; Bacteria; Bioinformatics; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Clouds; Community structure; Gene sequencing; Genomes; Genomics; Life Sciences; Microbial genetics; Microbiomes; Microorganisms; Scientific software; Sediment samplers; Sediments (Geology)

Individual microbes in metagenomic samples are sequenced by assembly of barcoded short reads Although shotgun metagenomic sequencing of microbiome samples enables partial reconstruction of strain-level community structure, obtaining high-quality microbial genome drafts without isolation and culture...