Knockout mutants of Actinobacillus pleuropneumoniae serotype 1 that are devoid of RTX toxins do not activate or kill porcine neutrophils

• Published in: Infection and Immunity Vol. 63; no. 1; pp. 27 - 37
• Main Authors: Jansen, R. (ID-DLO, Lelystad, The Netherlands.), Briaire, J, Smith, H.E, Dom, P, Haesebrouck, F, Kamp, E.M, Gielkens, L.J, Smits, M.A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.01.1995
• Subjects: ACTINOBACILLUS PLEUROPNEUMONIAE; Actinobacillus pleuropneumoniae - genetics; Actinobacillus pleuropneumoniae - immunology; Actinobacillus pleuropneumoniae - pathogenicity; Animal bacterial diseases; Animals; Bacterial diseases; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Bacterial Proteins - toxicity; Bacterial Toxins - biosynthesis; Bacterial Toxins - genetics; Bacterial Toxins - toxicity; Base Sequence; Biological and medical sciences; Cell Death; CERDO; Drug Resistance, Microbial - genetics; Electroporation; GENE; GENES; Genotype; GRANULOCITOS; GRANULOCYTE; Hemolysin Proteins; ID Lelystad, Institute for Animal Science and Health; ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid; Infectious diseases; Medical sciences; Molecular Sequence Data; MUTACION; Mutagenesis, Insertional; MUTANT; MUTANTES; MUTATION; Neutrophil Activation; OXIDACION; OXYDATION; PATHOGENESE; PATOGENESIS; Phenotype; PORCIN; PROTEINAS; PROTEINE; RECOMBINACION; RECOMBINAISON; Recombination, Genetic; Respiratory Burst; SECRECION; SECRETION; Swine; TOXICIDAD; TOXICITE; TOXINAS; TOXINE; Transformation, Genetic; Virulence - genetics; Lung disease; Pneumonia; Respiratory disease; Pathogenesis; Host agent relation; Pig; Infection; Pasteurellaceae; Toxin; Vertebrata; Mammalia; Bacteriosis; Bacteria; Artiodactyla; Mutation; Neutrophil; Actinobacillus pleuropneumoniae; Ungulata

The Actinobacillus pleuropneumoniae RTX-toxins ApxI, ApxII, and ApxIII are important virulence factors of this swine pathogen. It is hypothesized that the Apx toxins are deleterious to defense cells of the host, enabling the bacterium to infect the host. To confirm this, we studied the effect on por...