Accuracy of QuantiFERON SARS-CoV-2 research use only assay and characterization of the CD4+ and CD8+ T cell-SARS-CoV-2 response: comparison with a homemade interferon-γ release assay

• Published in: International journal of infectious diseases Vol. 122; pp. 841 - 849
• Main Authors: Aiello, Alessandra, Coppola, Andrea, Vanini, Valentina, Petrone, Linda, Cuzzi, Gilda, Salmi, Andrea, Altera, Anna Maria Gerarda, Tortorella, Carla, Gualano, Gina, Gasperini, Claudio, Scolieri, Palma, Beccacece, Alessia, Vita, Serena, Bruzzese, Vincenzo, Lorenzetti, Roberto, Palmieri, Fabrizio, Nicastri, Emanuele, Goletti, Delia
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.09.2022; The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases; Elsevier
• Subjects: CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; COVID-19; COVID-19 - diagnosis; Humans; IFN-γ release assay (IGRA), T cell response; Interferon-gamma Release Tests; Mycobacterium tuberculosis; QuantiFERON SARS-CoV-2 tubes; SARS-CoV-2; Spike peptides; Tuberculosis; Whole-blood; COVID-19; QuantiFERON SARS-CoV-2 tubes; Whole-blood; Spike peptides; IFN-γ release assay (IGRA), T cell response

•The QuantiFERON SARS-CoV-2 research use only assay response is mediated by CD4+ and CD8+ T cells.•Immune response to the antigen (Ag)2 tube is more accurate than that to the Ag1 tube.•Responses to QuantiFERON SARS-CoV-2 tubes are lower compared with a homemade test.•The QuantiFERON SARS-CoV-2 diffe...