An organoid model to assay the role of CFTR in the human epididymis epithelium

• Published in: Cell and tissue research Vol. 381; no. 2; pp. 327 - 336
• Main Authors: Leir, Shih-Hsing, Yin, Shiyi, Kerschner, Jenny L., Xia, Sunny, Ahmadi, Saumel, Bear, Christine, Harris, Ann
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2020; Springer; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Cell culture; Conductance; Cystic fibrosis; Dextran; Drug development; Epididymis; Epithelial cells; Epithelium; Forskolin; Gene expression; Human Genetics; Molecular Medicine; Organoids; Proteomics; Regular Article; Ribonucleic acid; RNA; RNA sequencing; siRNA; Vas deferens; Epididymis; Organoids; Single-cell RNA sequencing; Forskolin-induced swelling; Male reproductive tract

Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used hum...