An organoid model to assay the role of CFTR in the human epididymis epithelium

Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used hum...

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Published inCell and tissue research Vol. 381; no. 2; pp. 327 - 336
Main Authors Leir, Shih-Hsing, Yin, Shiyi, Kerschner, Jenny L., Xia, Sunny, Ahmadi, Saumel, Bear, Christine, Harris, Ann
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2020
Springer
Springer Nature B.V
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Abstract Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used human epididymis epithelial cell (HEE) organoids and polarized HEE cell cultures to assay the CF transmembrane conductance regulator (CFTR) in the human epididymis. 3D HEE organoids and polarized 2D HEE cell cultures on membrane inserts were established from human caput epididymis. Single-cell RNA sequencing (scRNA-seq) was performed to map cell type–specific gene expression in the organoids. Using forskolin (FSK) to activate CFTR and inhibitor CFTRinh 172 to block its activity, we assessed how CFTR contributes to organoid swelling and epithelial barrier function. The scRNA-seq data showed key caput epididymis cell types present in HEE organoid cultures. FSK at 10 μM induced HEE organoid swelling by 20% at 16 h, while 5 and 10 μM CFTRinh 172 treatment significantly reduced HEE organoid size. In transepithelial resistance (TER) measurements, FSK reduced TER, while inhibition of CFTR increased TER; also, depletion of CFTR with specific siRNAs significantly increased TER. FSK treatment significantly increased the flux of 4-kDa but not 70-kDa dextran, suggesting activation of CFTR mainly enhances transcellular diffusion. We have demonstrated that CFTR contributes to the maintenance of HEE cell TER and that cultured HEE organoids are a useful model to investigate human epididymis function. These results facilitate progress in elucidating how CFTR-dependent cellular processes impair fertility in CF.
AbstractList Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used human epididymis epithelial cell (HEE) organoids and polarized HEE cell cultures to assay the CF transmembrane conductance regulator (CFTR) in the human epididymis. 3D HEE organoids and polarized 2D HEE cell cultures on membrane inserts were established from human caput epididymis. Single-cell RNA sequencing (scRNA-seq) was performed to map cell type–specific gene expression in the organoids. Using forskolin (FSK) to activate CFTR and inhibitor CFTRinh 172 to block its activity, we assessed how CFTR contributes to organoid swelling and epithelial barrier function. The scRNA-seq data showed key caput epididymis cell types present in HEE organoid cultures. FSK at 10 μM induced HEE organoid swelling by 20% at 16 h, while 5 and 10 μM CFTRinh 172 treatment significantly reduced HEE organoid size. In transepithelial resistance (TER) measurements, FSK reduced TER, while inhibition of CFTR increased TER; also, depletion of CFTR with specific siRNAs significantly increased TER. FSK treatment significantly increased the flux of 4-kDa but not 70-kDa dextran, suggesting activation of CFTR mainly enhances transcellular diffusion. We have demonstrated that CFTR contributes to the maintenance of HEE cell TER and that cultured HEE organoids are a useful model to investigate human epididymis function. These results facilitate progress in elucidating how CFTR-dependent cellular processes impair fertility in CF.
Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used human epididymis epithelial cell (HEE) organoids and polarized HEE cell cultures to assay the CF transmembrane conductance regulator (CFTR) in the human epididymis. 3D HEE organoids and polarized 2D HEE cell cultures on membrane inserts were established from human caput epididymis. Single-cell RNA sequencing (scRNA-seq) was performed to map cell type–specific gene expression in the organoids. Using forskolin (FSK) to activate CFTR and inhibitor CFTRinh172 to block its activity, we assessed how CFTR contributes to organoid swelling and epithelial barrier function. The scRNA-seq data showed key caput epididymis cell types present in HEE organoid cultures. FSK at 10 μM induced HEE organoid swelling by 20% at 16 h, while 5 and 10 μM CFTRinh172 treatment significantly reduced HEE organoid size. In transepithelial resistance (TER) measurements, FSK reduced TER, while inhibition of CFTR increased TER; also, depletion of CFTR with specific siRNAs significantly increased TER. FSK treatment significantly increased the flux of 4-kDa but not 70-kDa dextran, suggesting activation of CFTR mainly enhances transcellular diffusion. We have demonstrated that CFTR contributes to the maintenance of HEE cell TER and that cultured HEE organoids are a useful model to investigate human epididymis function. These results facilitate progress in elucidating how CFTR-dependent cellular processes impair fertility in CF.
Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used human epididymis epithelial cell (HEE) organoids and polarized HEE cell cultures to assay the CF transmembrane conductance regulator (CFTR) in the human epididymis. 3D HEE organoids and polarized 2D HEE cell cultures on membrane inserts were established from human caput epididymis. Single-cell RNA sequencing (scRNA-seq) was performed to map cell type-specific gene expression in the organoids. Using forskolin (FSK) to activate CFTR and inhibitor CFTRinh.sub.172 to block its activity, we assessed how CFTR contributes to organoid swelling and epithelial barrier function. The scRNA-seq data showed key caput epididymis cell types present in HEE organoid cultures. FSK at 10 [mu]M induced HEE organoid swelling by 20% at 16 h, while 5 and 10 [mu]M CFTRinh.sub.172 treatment significantly reduced HEE organoid size. In transepithelial resistance (TER) measurements, FSK reduced TER, while inhibition of CFTR increased TER; also, depletion of CFTR with specific siRNAs significantly increased TER. FSK treatment significantly increased the flux of 4-kDa but not 70-kDa dextran, suggesting activation of CFTR mainly enhances transcellular diffusion. We have demonstrated that CFTR contributes to the maintenance of HEE cell TER and that cultured HEE organoids are a useful model to investigate human epididymis function. These results facilitate progress in elucidating how CFTR-dependent cellular processes impair fertility in CF.
Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used human epididymis epithelial cell (HEE) organoids and polarized HEE cell cultures to assay the CF transmembrane conductance regulator (CFTR) in the human epididymis. 3D HEE organoids and polarized 2D HEE cell cultures on membrane inserts were established from human caput epididymis. Single-cell RNA sequencing (scRNA-seq) was performed to map cell type-specific gene expression in the organoids. Using forskolin (FSK) to activate CFTR and inhibitor CFTRinh to block its activity, we assessed how CFTR contributes to organoid swelling and epithelial barrier function. The scRNA-seq data showed key caput epididymis cell types present in HEE organoid cultures. FSK at 10 μM induced HEE organoid swelling by 20% at 16 h, while 5 and 10 μM CFTRinh treatment significantly reduced HEE organoid size. In transepithelial resistance (TER) measurements, FSK reduced TER, while inhibition of CFTR increased TER; also, depletion of CFTR with specific siRNAs significantly increased TER. FSK treatment significantly increased the flux of 4-kDa but not 70-kDa dextran, suggesting activation of CFTR mainly enhances transcellular diffusion. We have demonstrated that CFTR contributes to the maintenance of HEE cell TER and that cultured HEE organoids are a useful model to investigate human epididymis function. These results facilitate progress in elucidating how CFTR-dependent cellular processes impair fertility in CF.
Organoid cultures derived from primary human tissues facilitate the study of disease processes and the development of new therapeutics. Most men with cystic fibrosis (CF) are infertile due to defects in the epididymis and vas deferens; however, the causative mechanisms are still unclear. We used human epididymis epithelial cell (HEE) organoids and polarized HEE cell cultures to assay the CF transmembrane conductance regulator (CFTR) in the human epididymis. 3D HEE organoids and polarized 2D HEE cell cultures on membrane inserts were established from human caput epididymis. Single-cell RNA sequencing (scRNA-seq) was performed to map cell-type-specific gene expression in the organoids. Using forskolin (FSK) to activate CFTR and inhibitor CFTRinh 172 to block its activity, we assessed how CFTR contributes to organoid swelling and epithelial barrier function. The scRNA-seq data showed key caput epididymis cell types present in HEE organoid cultures. FSK at 10 μM induced HEE organoid swelling by 20% at 16 hrs, while 5 and 10 μM CFTRinh 172 treatment significantly reduced HEE organoid size. In transepithelial resistance (TER) measurements, FSK reduced TER, while inhibition of CFTR increased TER; also, depletion of CFTR with specific siRNAs significantly increased TER. FSK treatment significantly increased the flux of 4-kDa but not 70kDa dextran, suggesting activation of CFTR mainly enhances transcellular diffusion. We have demonstrated that CFTR contributes to the maintenance of HEE cell TER and that cultured HEE organoids are a useful model to investigate human epididymis function. This research facilitates progress in elucidating how CFTR-dependent cellular processes impair fertility in CF.
Audience Academic
Author Leir, Shih-Hsing
Ahmadi, Saumel
Kerschner, Jenny L.
Harris, Ann
Yin, Shiyi
Bear, Christine
Xia, Sunny
AuthorAffiliation 1 Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, OH 44106, USA
2 Molecular Medicine, Research Institute at the Hospital for Sick Children, Physiology and Biochemistry at the University of Toronto, Toronto, ON M5G 1X8, Canada
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  surname: Harris
  fullname: Harris, Ann
  organization: Department of Genetics and Genome Sciences, Case Western Reserve University
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32377875$$D View this record in MEDLINE/PubMed
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Cites_doi 10.1126/science.1704151
10.1016/j.stem.2013.11.002
10.1177/2211068214561025
10.1093/molehr/gav066
10.1093/molehr/gaq008
10.1172/JCI0216112
10.1186/1471-2164-10-369
10.1038/nature07935
10.1016/j.jcf.2008.05.003
10.1016/S1046-2023(03)00029-X
10.1042/BCJ20180044
10.1095/biolreprod.115.133967
10.1101/cshperspect.a009498
10.21451/1984-3143-AR955
10.3389/fendo.2018.00059
10.1111/bph.12726
10.1053/j.gastro.2011.07.050
10.1530/REP-13-0420
10.1111/andr.12635
10.1002/(SICI)1098-2795(200002)55:2<125::AID-MRD1>3.0.CO;2-Q
10.1111/rda.13082
10.1085/jgp.200810122
10.3389/fphar.2018.01429
10.1126/scitranslmed.aad8278
10.1242/jcs.148098
10.1111/andr.12629
10.1093/humupd/dmn055
10.1093/hmg/2.3.213
10.1016/S0140-6736(95)91431-5
10.1016/j.cell.2016.05.082
10.1039/b924455g
10.1073/pnas.88.20.9262
10.1038/nbt.3294
10.1093/humupd/dmw015
10.1038/sj.emboj.7601387
10.1073/pnas.0609253104
10.1007/978-1-4615-0679-9
10.15252/embj.2018100300
10.2164/jandrol.107.004119
10.1086/301606
10.5772/35847
10.1095/biolreprod.111.090860
10.1056/NEJM197209212871204
10.1038/nm.3201
10.1095/biolreprod.109.083196
10.1016/S0015-0282(16)55327-8
10.1111/andr.12633
ContentType Journal Article
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Issue 2
Keywords Epididymis
Organoids
Single-cell RNA sequencing
Forskolin-induced swelling
Male reproductive tract
Language English
License Terms of use and reuse: academic research for non-commercial purposes, see here for full terms. http://www.springer.com/gb/open-access/authors-rights/aam-terms-v1
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References Zhou, De Iuliis, Dun, Nixon (CR48) 2018; 9
Leir, Browne, Eggener, Harris (CR18) 2015; 103
Sato (CR36) 2011; 141
Srinivasan, Kolli, Esch, Abaci, Shuler, Hickman (CR39) 2015; 20
Browne, Yang, Leir, Eggener, Harris (CR4) 2016; 22
Cornwall (CR7) 2009; 15
Turner (CR44) 2008; 29
Schwank (CR37) 2013; 13
Sullivan, Legare, Lamontagne-Proulx, Breton, Soulet (CR41) 2019; 7
Taussig, Lobeck, di Sant'Agnese, Ackerman, Kattwinkel (CR42) 1972; 287
Sullivan, Mieusset (CR40) 2016; 22
Xu (CR46) 2007; 104
Dekkers (CR10) 2013; 19
Li, Naren (CR19) 2010; 2
Moran, Zegarra-Moran (CR26) 2008; 7
Dodge (CR12) 1995; 346
Moore, Curry, Penfold, Pryor (CR25) 1992; 58
Melis, Tauc, Cougnon, Bendahhou, Giuliano, Rubera, Duranton (CR23) 2014; 171
Awatade, Wong, Hewson, Fawcett, Kicic, Jaffe, Waters (CR3) 2018; 9
Ma, Thiagarajah, Yang, Sonawane, Folli, Galietta, Verkman (CR20) 2002; 110
Mutolo, Leir, Fossum, Browne, Harris (CR27) 2018; 475
CR2
Kristensen (CR17) 2010; 16
Ruan (CR33) 2014; 127
Trezise, Chambers, Wardle, Gould, Harris (CR43) 1993; 2
Ogawa (CR28) 2015; 33
Pinel, Mandon, Cyr (CR30) 2019; 7
Dekkers (CR11) 2016; 8
Sachs (CR34) 2019; 38
Dube, Dufresne, Chan, Hermo, Cyr (CR13) 2010; 82
Pierucci-Alves, Akoyev, Stewart, Wang, Janardhan, Schultz (CR29) 2011; 85
Browne, Leir, Yin, Harris (CR5) 2019; 7
Sato (CR35) 2009; 459
Yeste, Morato, Rodriguez-Gil, Bonet, Prieto-Martinez (CR47) 2017; 52
Anderson, Rich, Gregory, Smith, Welsh (CR1) 1991; 251
Crawford (CR8) 1991; 88
Ishiguro (CR16) 2009; 133
Meschede, Dworniczak, Behre, Kliesch, Claustres, Nieschlag, Horst (CR24) 1997; 61
Hwang, Kirk (CR15) 2013; 3
Reynaert, Van Der Schueren, Degeest, Manin, Cuppens, Scholte, Cassiman (CR31) 2000; 55
Robaire, Hinton (CR32) 2002
Guyonnet, Marot, Dacheux, Mercat, Schwob, Jaffrezic, Gatti (CR14) 2009; 10
Souza, Lenz, Lucca De Lazari, Bustamante-Filho (CR38) 2017; 14
Dacheux, Dacheux (CR9) 2014; 147
Mandon, Hermo, Cyr (CR21) 2015; 93
Wang (CR45) 2006; 25
Clevers (CR6) 2016; 165
Matter, Balda (CR22) 2003; 30
JA Browne (3208_CR4) 2016; 22
AE Trezise (3208_CR43) 1993; 2
LM Taussig (3208_CR42) 1972; 287
N Sachs (3208_CR34) 2019; 38
DM Kristensen (3208_CR17) 2010; 16
K Matter (3208_CR22) 2003; 30
C Li (3208_CR19) 2010; 2
G Schwank (3208_CR37) 2013; 13
WM Xu (3208_CR46) 2007; 104
TC Hwang (3208_CR15) 2013; 3
M Mandon (3208_CR21) 2015; 93
O Moran (3208_CR26) 2008; 7
I Crawford (3208_CR8) 1991; 88
F Pierucci-Alves (3208_CR29) 2011; 85
JL Dacheux (3208_CR9) 2014; 147
I Reynaert (3208_CR31) 2000; 55
GA Cornwall (3208_CR7) 2009; 15
JF Dekkers (3208_CR11) 2016; 8
B Guyonnet (3208_CR14) 2009; 10
HD Moore (3208_CR25) 1992; 58
M Yeste (3208_CR47) 2017; 52
NT Awatade (3208_CR3) 2018; 9
T Sato (3208_CR36) 2011; 141
H Ishiguro (3208_CR16) 2009; 133
TT Turner (3208_CR44) 2008; 29
N Melis (3208_CR23) 2014; 171
T Ma (3208_CR20) 2002; 110
MJ Mutolo (3208_CR27) 2018; 475
W Zhou (3208_CR48) 2018; 9
A Souza (3208_CR38) 2017; 14
H Clevers (3208_CR6) 2016; 165
R Sullivan (3208_CR41) 2019; 7
B Srinivasan (3208_CR39) 2015; 20
YC Ruan (3208_CR33) 2014; 127
M Ogawa (3208_CR28) 2015; 33
3208_CR2
JF Dekkers (3208_CR10) 2013; 19
JA Browne (3208_CR5) 2019; 7
D Meschede (3208_CR24) 1997; 61
MP Anderson (3208_CR1) 1991; 251
E Dube (3208_CR13) 2010; 82
SH Leir (3208_CR18) 2015; 103
R Sullivan (3208_CR40) 2016; 22
JA Dodge (3208_CR12) 1995; 346
B Robaire (3208_CR32) 2002
Y Wang (3208_CR45) 2006; 25
T Sato (3208_CR35) 2009; 459
L Pinel (3208_CR30) 2019; 7
References_xml – volume: 346
  start-page: 587
  year: 1995
  end-page: 588
  ident: CR12
  article-title: Male fertility in cystic fibrosis
  publication-title: Lancet
  contributor:
    fullname: Dodge
– volume: 61
  start-page: 1200
  year: 1997
  end-page: 1202
  ident: CR24
  article-title: CFTR gene mutations in men with bilateral ejaculatory-duct obstruction and anomalies of the seminal vesicles
  publication-title: Am J Hum Genet
  contributor:
    fullname: Horst
– volume: 9
  start-page: 1429
  year: 2018
  ident: CR3
  article-title: Human primary epithelial cell models: promising tools in the era of cystic fibrosis personalized medicine
  publication-title: Front Pharmacol
  contributor:
    fullname: Waters
– volume: 459
  start-page: 262
  year: 2009
  end-page: 265
  ident: CR35
  article-title: Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
  publication-title: Nature
  contributor:
    fullname: Sato
– volume: 133
  start-page: 315
  year: 2009
  end-page: 326
  ident: CR16
  article-title: CFTR functions as a bicarbonate channel in pancreatic duct cells
  publication-title: J Gen Physiol
  contributor:
    fullname: Ishiguro
– volume: 88
  start-page: 9262
  year: 1991
  end-page: 9266
  ident: CR8
  article-title: Immunocytochemical localization of the cystic fibrosis gene product CFTR
  publication-title: Proc Natl Acad Sci U S A
  contributor:
    fullname: Crawford
– volume: 15
  start-page: 213
  year: 2009
  end-page: 227
  ident: CR7
  article-title: New insights into epididymal biology and function
  publication-title: Hum Reprod Update
  contributor:
    fullname: Cornwall
– year: 2002
  ident: CR32
  publication-title: The epididymis: from molecules to clinical practice
  contributor:
    fullname: Hinton
– ident: CR2
– volume: 14
  start-page: 1234
  year: 2017
  end-page: 1244
  ident: CR38
  article-title: The epididymis and its role on sperm quality and male fertility
  publication-title: Anim Reprod
  contributor:
    fullname: Bustamante-Filho
– volume: 165
  start-page: 1586
  year: 2016
  end-page: 1597
  ident: CR6
  article-title: Modeling development and disease with organoids
  publication-title: Cell
  contributor:
    fullname: Clevers
– volume: 7
  start-page: 741
  year: 2019
  end-page: 747
  ident: CR5
  article-title: Transcriptional networks in the human epididymis
  publication-title: Andrology
  contributor:
    fullname: Harris
– volume: 103
  issue: 647–654
  year: 2015
  ident: CR18
  article-title: Characterization of primary cultures of adult human epididymis epithelial cells
  publication-title: Fertil Steril
  contributor:
    fullname: Harris
– volume: 2
  start-page: 161
  year: 2010
  end-page: 177
  ident: CR19
  article-title: CFTR chloride channel in the apical compartments: spatiotemporal coupling to its interacting partners
  publication-title: Integr Biol (Camb)
  contributor:
    fullname: Naren
– volume: 3
  start-page: a009498
  year: 2013
  ident: CR15
  article-title: The CFTR ion channel: gating, regulation, and anion permeation
  publication-title: Cold Spring Harb Perspect Med
  contributor:
    fullname: Kirk
– volume: 55
  start-page: 125
  year: 2000
  end-page: 135
  ident: CR31
  article-title: Morphological changes in the vas deferens and expression of the cystic fibrosis transmembrane conductance regulator (CFTR) in control, deltaF508 and knock-out CFTR mice during postnatal life
  publication-title: Mol Reprod Dev
  contributor:
    fullname: Cassiman
– volume: 25
  start-page: 5049
  year: 2006
  end-page: 5057
  ident: CR45
  article-title: Slc26a6 regulates CFTR activity in vivo to determine pancreatic duct HCO3- secretion: relevance to cystic fibrosis
  publication-title: EMBO J
  contributor:
    fullname: Wang
– volume: 171
  start-page: 3716
  year: 2014
  end-page: 3727
  ident: CR23
  article-title: Revisiting CFTR inhibition: a comparative study of CFTRinh -172 and GlyH-101 inhibitors
  publication-title: Br J Pharmacol
  contributor:
    fullname: Duranton
– volume: 7
  start-page: 618
  year: 2019
  end-page: 630
  ident: CR30
  article-title: Tissue regeneration and the epididymal stem cell
  publication-title: Andrology
  contributor:
    fullname: Cyr
– volume: 2
  start-page: 213
  year: 1993
  end-page: 218
  ident: CR43
  article-title: Expression of the cystic fibrosis gene in human foetal tissues
  publication-title: Hum Mol Genet
  contributor:
    fullname: Harris
– volume: 38
  year: 2019
  ident: CR34
  article-title: Long-term expanding human airway organoids for disease modeling
  publication-title: EMBO J
  contributor:
    fullname: Sachs
– volume: 82
  start-page: 1119
  year: 2010
  end-page: 1128
  ident: CR13
  article-title: Assessing the role of claudins in maintaining the integrity of epididymal tight junctions using novel human epididymal cell lines
  publication-title: Biol Reprod
  contributor:
    fullname: Cyr
– volume: 33
  start-page: 853
  year: 2015
  end-page: 861
  ident: CR28
  article-title: Directed differentiation of cholangiocytes from human pluripotent stem cells
  publication-title: Nat Biotechnol
  contributor:
    fullname: Ogawa
– volume: 7
  start-page: 748
  year: 2019
  end-page: 757
  ident: CR41
  article-title: Revisiting structure/functions of the human epididymis
  publication-title: Andrology
  contributor:
    fullname: Soulet
– volume: 58
  start-page: 776
  year: 1992
  end-page: 783
  ident: CR25
  article-title: The culture of human epididymal epithelium and in vitro maturation of epididymal spermatozoa
  publication-title: Fertil Steril
  contributor:
    fullname: Pryor
– volume: 141
  start-page: 1762
  year: 2011
  end-page: 1772
  ident: CR36
  article-title: Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium
  publication-title: Gastroenterology
  contributor:
    fullname: Sato
– volume: 13
  start-page: 653
  year: 2013
  end-page: 658
  ident: CR37
  article-title: Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients
  publication-title: Cell Stem Cell
  contributor:
    fullname: Schwank
– volume: 287
  start-page: 586
  year: 1972
  end-page: 589
  ident: CR42
  article-title: Fertility in males with cystic fibrosis
  publication-title: N Engl J Med
  contributor:
    fullname: Kattwinkel
– volume: 19
  start-page: 939
  year: 2013
  end-page: 945
  ident: CR10
  article-title: A functional CFTR assay using primary cystic fibrosis intestinal organoids
  publication-title: Nat Med
  contributor:
    fullname: Dekkers
– volume: 10
  start-page: 369
  year: 2009
  ident: CR14
  article-title: The adult boar testicular and epididymal transcriptomes
  publication-title: BMC Genomics
  contributor:
    fullname: Gatti
– volume: 85
  start-page: 442
  year: 2011
  end-page: 451
  ident: CR29
  article-title: Swine models of cystic fibrosis reveal male reproductive tract phenotype at birth
  publication-title: Biol Reprod
  contributor:
    fullname: Schultz
– volume: 127
  start-page: 4396
  year: 2014
  end-page: 4408
  ident: CR33
  article-title: CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation through the ZONAB pathway
  publication-title: J Cell Sci
  contributor:
    fullname: Ruan
– volume: 16
  start-page: 835
  year: 2010
  end-page: 845
  ident: CR17
  article-title: OCT4 and downstream factors are expressed in human somatic urogenital epithelia and in culture of epididymal spheres
  publication-title: Mol Hum Reprod
  contributor:
    fullname: Kristensen
– volume: 22
  start-page: 574
  year: 2016
  end-page: 587
  ident: CR40
  article-title: The human epididymis: its function in sperm maturation
  publication-title: Hum Reprod Update
  contributor:
    fullname: Mieusset
– volume: 9
  start-page: 59
  year: 2018
  ident: CR48
  article-title: Characteristics of the epididymal luminal environment responsible for sperm maturation and storage
  publication-title: Front Endocrinol (Lausanne)
  contributor:
    fullname: Nixon
– volume: 30
  start-page: 228
  year: 2003
  end-page: 234
  ident: CR22
  article-title: Functional analysis of tight junctions
  publication-title: Methods
  contributor:
    fullname: Balda
– volume: 7
  start-page: 483
  year: 2008
  end-page: 494
  ident: CR26
  article-title: On the measurement of the functional properties of the CFTR
  publication-title: J Cyst Fibros
  contributor:
    fullname: Zegarra-Moran
– volume: 475
  start-page: 1323
  year: 2018
  end-page: 1334
  ident: CR27
  article-title: A transcription factor network represses CFTR gene expression in airway epithelial cells
  publication-title: Biochem J
  contributor:
    fullname: Harris
– volume: 104
  start-page: 9816
  year: 2007
  end-page: 9821
  ident: CR46
  article-title: Cystic fibrosis transmembrane conductance regulator is vital to sperm fertilizing capacity and male fertility
  publication-title: Proc Natl Acad Sci U S A
  contributor:
    fullname: Xu
– volume: 29
  start-page: 237
  year: 2008
  end-page: 250
  ident: CR44
  article-title: De Graaf’s thread: the human epididymis
  publication-title: J Androl
  contributor:
    fullname: Turner
– volume: 251
  start-page: 679
  year: 1991
  end-page: 682
  ident: CR1
  article-title: Generation of cAMP-activated chloride currents by expression of CFTR
  publication-title: Science
  contributor:
    fullname: Welsh
– volume: 22
  start-page: 69
  year: 2016
  end-page: 82
  ident: CR4
  article-title: Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions
  publication-title: Mol Hum Reprod
  contributor:
    fullname: Harris
– volume: 110
  start-page: 1651
  year: 2002
  end-page: 1658
  ident: CR20
  article-title: Thiazolidinone CFTR inhibitor identified by high-throughput screening blocks cholera toxin-induced intestinal fluid secretion
  publication-title: J Clin Invest
  contributor:
    fullname: Verkman
– volume: 20
  start-page: 107
  year: 2015
  end-page: 126
  ident: CR39
  article-title: TEER measurement techniques for in vitro barrier model systems
  publication-title: J Lab Autom
  contributor:
    fullname: Hickman
– volume: 52
  start-page: 12
  issue: Suppl 4
  year: 2017
  end-page: 27
  ident: CR47
  article-title: Aquaporins in the male reproductive tract and sperm: functional implications and cryobiology
  publication-title: Reprod Domest Anim
  contributor:
    fullname: Prieto-Martinez
– volume: 93
  start-page: 115
  year: 2015
  ident: CR21
  article-title: Isolated rat epididymal basal cells share common properties with adult stem cells
  publication-title: Biol Reprod
  contributor:
    fullname: Cyr
– volume: 147
  start-page: R27
  year: 2014
  end-page: R42
  ident: CR9
  article-title: New insights into epididymal function in relation to sperm maturation
  publication-title: Reproduction
  contributor:
    fullname: Dacheux
– volume: 8
  start-page: 344ra384
  year: 2016
  ident: CR11
  article-title: Characterizing responses to CFTR-modulating drugs using rectal organoids derived from subjects with cystic fibrosis
  publication-title: Sci Transl Med
  contributor:
    fullname: Dekkers
– volume: 251
  start-page: 679
  year: 1991
  ident: 3208_CR1
  publication-title: Science
  doi: 10.1126/science.1704151
  contributor:
    fullname: MP Anderson
– volume: 13
  start-page: 653
  year: 2013
  ident: 3208_CR37
  publication-title: Cell Stem Cell
  doi: 10.1016/j.stem.2013.11.002
  contributor:
    fullname: G Schwank
– volume: 20
  start-page: 107
  year: 2015
  ident: 3208_CR39
  publication-title: J Lab Autom
  doi: 10.1177/2211068214561025
  contributor:
    fullname: B Srinivasan
– volume: 103
  issue: 647–654
  year: 2015
  ident: 3208_CR18
  publication-title: Fertil Steril
  contributor:
    fullname: SH Leir
– volume: 22
  start-page: 69
  year: 2016
  ident: 3208_CR4
  publication-title: Mol Hum Reprod
  doi: 10.1093/molehr/gav066
  contributor:
    fullname: JA Browne
– volume: 16
  start-page: 835
  year: 2010
  ident: 3208_CR17
  publication-title: Mol Hum Reprod
  doi: 10.1093/molehr/gaq008
  contributor:
    fullname: DM Kristensen
– volume: 110
  start-page: 1651
  year: 2002
  ident: 3208_CR20
  publication-title: J Clin Invest
  doi: 10.1172/JCI0216112
  contributor:
    fullname: T Ma
– volume: 10
  start-page: 369
  year: 2009
  ident: 3208_CR14
  publication-title: BMC Genomics
  doi: 10.1186/1471-2164-10-369
  contributor:
    fullname: B Guyonnet
– volume: 459
  start-page: 262
  year: 2009
  ident: 3208_CR35
  publication-title: Nature
  doi: 10.1038/nature07935
  contributor:
    fullname: T Sato
– volume: 7
  start-page: 483
  year: 2008
  ident: 3208_CR26
  publication-title: J Cyst Fibros
  doi: 10.1016/j.jcf.2008.05.003
  contributor:
    fullname: O Moran
– volume: 30
  start-page: 228
  year: 2003
  ident: 3208_CR22
  publication-title: Methods
  doi: 10.1016/S1046-2023(03)00029-X
  contributor:
    fullname: K Matter
– volume: 475
  start-page: 1323
  year: 2018
  ident: 3208_CR27
  publication-title: Biochem J
  doi: 10.1042/BCJ20180044
  contributor:
    fullname: MJ Mutolo
– volume: 93
  start-page: 115
  year: 2015
  ident: 3208_CR21
  publication-title: Biol Reprod
  doi: 10.1095/biolreprod.115.133967
  contributor:
    fullname: M Mandon
– volume: 3
  start-page: a009498
  year: 2013
  ident: 3208_CR15
  publication-title: Cold Spring Harb Perspect Med
  doi: 10.1101/cshperspect.a009498
  contributor:
    fullname: TC Hwang
– volume: 14
  start-page: 1234
  year: 2017
  ident: 3208_CR38
  publication-title: Anim Reprod
  doi: 10.21451/1984-3143-AR955
  contributor:
    fullname: A Souza
– volume: 9
  start-page: 59
  year: 2018
  ident: 3208_CR48
  publication-title: Front Endocrinol (Lausanne)
  doi: 10.3389/fendo.2018.00059
  contributor:
    fullname: W Zhou
– volume: 171
  start-page: 3716
  year: 2014
  ident: 3208_CR23
  publication-title: Br J Pharmacol
  doi: 10.1111/bph.12726
  contributor:
    fullname: N Melis
– volume: 141
  start-page: 1762
  year: 2011
  ident: 3208_CR36
  publication-title: Gastroenterology
  doi: 10.1053/j.gastro.2011.07.050
  contributor:
    fullname: T Sato
– volume: 147
  start-page: R27
  year: 2014
  ident: 3208_CR9
  publication-title: Reproduction
  doi: 10.1530/REP-13-0420
  contributor:
    fullname: JL Dacheux
– volume: 7
  start-page: 618
  year: 2019
  ident: 3208_CR30
  publication-title: Andrology
  doi: 10.1111/andr.12635
  contributor:
    fullname: L Pinel
– volume: 55
  start-page: 125
  year: 2000
  ident: 3208_CR31
  publication-title: Mol Reprod Dev
  doi: 10.1002/(SICI)1098-2795(200002)55:2<125::AID-MRD1>3.0.CO;2-Q
  contributor:
    fullname: I Reynaert
– volume: 52
  start-page: 12
  issue: Suppl 4
  year: 2017
  ident: 3208_CR47
  publication-title: Reprod Domest Anim
  doi: 10.1111/rda.13082
  contributor:
    fullname: M Yeste
– volume: 133
  start-page: 315
  year: 2009
  ident: 3208_CR16
  publication-title: J Gen Physiol
  doi: 10.1085/jgp.200810122
  contributor:
    fullname: H Ishiguro
– volume: 9
  start-page: 1429
  year: 2018
  ident: 3208_CR3
  publication-title: Front Pharmacol
  doi: 10.3389/fphar.2018.01429
  contributor:
    fullname: NT Awatade
– volume: 8
  start-page: 344ra384
  year: 2016
  ident: 3208_CR11
  publication-title: Sci Transl Med
  doi: 10.1126/scitranslmed.aad8278
  contributor:
    fullname: JF Dekkers
– volume: 127
  start-page: 4396
  year: 2014
  ident: 3208_CR33
  publication-title: J Cell Sci
  doi: 10.1242/jcs.148098
  contributor:
    fullname: YC Ruan
– volume: 7
  start-page: 741
  year: 2019
  ident: 3208_CR5
  publication-title: Andrology
  doi: 10.1111/andr.12629
  contributor:
    fullname: JA Browne
– volume: 15
  start-page: 213
  year: 2009
  ident: 3208_CR7
  publication-title: Hum Reprod Update
  doi: 10.1093/humupd/dmn055
  contributor:
    fullname: GA Cornwall
– volume: 2
  start-page: 213
  year: 1993
  ident: 3208_CR43
  publication-title: Hum Mol Genet
  doi: 10.1093/hmg/2.3.213
  contributor:
    fullname: AE Trezise
– volume: 346
  start-page: 587
  year: 1995
  ident: 3208_CR12
  publication-title: Lancet
  doi: 10.1016/S0140-6736(95)91431-5
  contributor:
    fullname: JA Dodge
– volume: 165
  start-page: 1586
  year: 2016
  ident: 3208_CR6
  publication-title: Cell
  doi: 10.1016/j.cell.2016.05.082
  contributor:
    fullname: H Clevers
– volume: 2
  start-page: 161
  year: 2010
  ident: 3208_CR19
  publication-title: Integr Biol (Camb)
  doi: 10.1039/b924455g
  contributor:
    fullname: C Li
– volume: 88
  start-page: 9262
  year: 1991
  ident: 3208_CR8
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.88.20.9262
  contributor:
    fullname: I Crawford
– volume: 33
  start-page: 853
  year: 2015
  ident: 3208_CR28
  publication-title: Nat Biotechnol
  doi: 10.1038/nbt.3294
  contributor:
    fullname: M Ogawa
– volume: 22
  start-page: 574
  year: 2016
  ident: 3208_CR40
  publication-title: Hum Reprod Update
  doi: 10.1093/humupd/dmw015
  contributor:
    fullname: R Sullivan
– volume: 25
  start-page: 5049
  year: 2006
  ident: 3208_CR45
  publication-title: EMBO J
  doi: 10.1038/sj.emboj.7601387
  contributor:
    fullname: Y Wang
– volume: 104
  start-page: 9816
  year: 2007
  ident: 3208_CR46
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.0609253104
  contributor:
    fullname: WM Xu
– volume-title: The epididymis: from molecules to clinical practice
  year: 2002
  ident: 3208_CR32
  doi: 10.1007/978-1-4615-0679-9
  contributor:
    fullname: B Robaire
– volume: 38
  year: 2019
  ident: 3208_CR34
  publication-title: EMBO J
  doi: 10.15252/embj.2018100300
  contributor:
    fullname: N Sachs
– volume: 29
  start-page: 237
  year: 2008
  ident: 3208_CR44
  publication-title: J Androl
  doi: 10.2164/jandrol.107.004119
  contributor:
    fullname: TT Turner
– volume: 61
  start-page: 1200
  year: 1997
  ident: 3208_CR24
  publication-title: Am J Hum Genet
  doi: 10.1086/301606
  contributor:
    fullname: D Meschede
– ident: 3208_CR2
  doi: 10.5772/35847
– volume: 85
  start-page: 442
  year: 2011
  ident: 3208_CR29
  publication-title: Biol Reprod
  doi: 10.1095/biolreprod.111.090860
  contributor:
    fullname: F Pierucci-Alves
– volume: 287
  start-page: 586
  year: 1972
  ident: 3208_CR42
  publication-title: N Engl J Med
  doi: 10.1056/NEJM197209212871204
  contributor:
    fullname: LM Taussig
– volume: 19
  start-page: 939
  year: 2013
  ident: 3208_CR10
  publication-title: Nat Med
  doi: 10.1038/nm.3201
  contributor:
    fullname: JF Dekkers
– volume: 82
  start-page: 1119
  year: 2010
  ident: 3208_CR13
  publication-title: Biol Reprod
  doi: 10.1095/biolreprod.109.083196
  contributor:
    fullname: E Dube
– volume: 58
  start-page: 776
  year: 1992
  ident: 3208_CR25
  publication-title: Fertil Steril
  doi: 10.1016/S0015-0282(16)55327-8
  contributor:
    fullname: HD Moore
– volume: 7
  start-page: 748
  year: 2019
  ident: 3208_CR41
  publication-title: Andrology
  doi: 10.1111/andr.12633
  contributor:
    fullname: R Sullivan
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SubjectTerms Biomedical and Life Sciences
Biomedicine
Cell culture
Conductance
Cystic fibrosis
Dextran
Drug development
Epididymis
Epithelial cells
Epithelium
Forskolin
Gene expression
Human Genetics
Molecular Medicine
Organoids
Proteomics
Regular Article
Ribonucleic acid
RNA
RNA sequencing
siRNA
Vas deferens
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Title An organoid model to assay the role of CFTR in the human epididymis epithelium
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