TroA of Streptococcus suis Is Required for Manganese Acquisition and Full Virulence

• Published in: Journal of Bacteriology Vol. 193; no. 19; pp. 5073 - 5080
• Main Authors: Schreur, Paul J. Wichgers, Rebel, Johanna M. J, Smits, Mari A, van Putten, Jos P. M, Smith, Hilde E
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.2011
• Subjects: 7 european countries; Animals; arthritis; bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; blood serum; copper; crystal-structure; diseased pigs; Environmental regulations; Enzymes and Proteins; Female; Fundamental and applied biological sciences. Psychology; Gene expression; genes; Gram-positive bacteria; hydrogen peroxide; Hydrogen Peroxide - pharmacology; infection; iron; lipoproteins; Magnesium; Manganese; Manganese - metabolism; Manganese compounds; meningitis; Mice; Microbiology; Miscellaneous; Mutants; Mutation; Nickel; nutrients; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - genetics; phenotype; pneumococcal surface-antigen; pneumoniae; protein; Proteins; septic shock; serotype-2; Streptococcal Infections - genetics; Streptococcal Infections - microbiology; Streptococcus suis; Streptococcus suis - genetics; Streptococcus suis - metabolism; Streptococcus suis - pathogenicity; swine; Trace metals; vaccine; virulence; Virulence - genetics; Virulence - physiology; zinc; Streptococcaceae; Virulence; Streptococcus suis; Bacteria; Micrococcales; Heavy metal; Manganese
• ISSN: 0021-9193; 1098-5530; 1098-5530; 1067-8832
• DOI: 10.1128/JB.05305-11

Streptococcus suis causes infections in pigs and occasionally in humans, resulting in manifestations as meningitis, sepsis, arthritis, and septic shock. For survival within the host, S. suis requires numerous nutrients including trace metals. Little is known about the specific proteins involved in metal scavenging in S. suis. In this study we evaluated the role of the putative high-affinity metal binding lipoprotein TroA in metal acquisition and virulence. A mutant strain deficient in the expression of TroA (ΔtroA mutant) was constructed. Growth of the ΔtroA mutant in Todd-Hewitt broth was similar to wild-type growth; however, growth of the ΔtroA mutant in cation-deprived Todd-Hewitt broth and in porcine serum was strongly reduced compared to growth of wild-type bacteria. Supplementing the medium with extra manganese but not with magnesium, zinc, copper, nickel, or iron restored growth to wild-type levels, indicating that TroA is specifically required for growth in environments low in manganese. The ΔtroA mutant also showed increased susceptibility to H2O2, suggesting that TroA is involved in counteracting oxidative stress. Furthermore, the expression of the troA gene was subject to environmental regulation at the transcript level. In a murine S. suis infection model, the ΔtroA mutant displayed a nonvirulent phenotype. These data indicate that S. suis TroA is involved in manganese acquisition and is required for full virulence in mice.