Identification of a Devernalization Inducer by Chemical Screening Approaches in Arabidopsis thaliana
Vernalization is the promotion of flowering after prolonged exposure to cold. In , vernalization induces epigenetic silencing of the floral repressor gene ( ). The repressive epigenetic mark trimethylation of lysine 27 on histone H3 proteins (H3K27me3) is a critical contributor to the epigenetic sil...
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| Published in | Frontiers in plant science Vol. 12; p. 634068 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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| Abstract | Vernalization is the promotion of flowering after prolonged exposure to cold. In
, vernalization induces epigenetic silencing of the floral repressor gene
(
). The repressive epigenetic mark trimethylation of lysine 27 on histone H3 proteins (H3K27me3) is a critical contributor to the epigenetic silencing of
. Interestingly, the deposited H3K27me3 in the
locus can be erased by short-term high-temperature treatment. This is referred to as devernalization. In this study, we identified a novel chemical compound, 4-Isoxazolecarboxylic acid, 3,5-dimethyl-2-(4-fluorophenyl)-4-isoxazole carboxylic acid 1-methyl-2-oxoethyl ester named as DEVERNALIZER01 (DVR01), which induces devernalization in
seedlings, by an
-luciferase reporter-based high-throughput screening assay. DVR01 decreased the amount of H3K27me3 in the
locus in vernalized plants, resulting in the upregulation of
in the whole plant, including the vasculature and meristem, where FLC represses floral induction genes. We also showed that a 2-week treatment with DVR01 reverted plants with a vernalized status back to a fully non-vernalized status. Collectively, this study provides a novel structure of DVR01, which modulates devernalization via demethylation of H3K27me3 in the
locus. |
|---|---|
| AbstractList | Vernalization is the promotion of flowering after prolonged exposure to cold. In
, vernalization induces epigenetic silencing of the floral repressor gene
(
). The repressive epigenetic mark trimethylation of lysine 27 on histone H3 proteins (H3K27me3) is a critical contributor to the epigenetic silencing of
. Interestingly, the deposited H3K27me3 in the
locus can be erased by short-term high-temperature treatment. This is referred to as devernalization. In this study, we identified a novel chemical compound, 4-Isoxazolecarboxylic acid, 3,5-dimethyl-2-(4-fluorophenyl)-4-isoxazole carboxylic acid 1-methyl-2-oxoethyl ester named as DEVERNALIZER01 (DVR01), which induces devernalization in
seedlings, by an
-luciferase reporter-based high-throughput screening assay. DVR01 decreased the amount of H3K27me3 in the
locus in vernalized plants, resulting in the upregulation of
in the whole plant, including the vasculature and meristem, where FLC represses floral induction genes. We also showed that a 2-week treatment with DVR01 reverted plants with a vernalized status back to a fully non-vernalized status. Collectively, this study provides a novel structure of DVR01, which modulates devernalization via demethylation of H3K27me3 in the
locus. Vernalization is the promotion of flowering after prolonged exposure to cold. In Arabidopsis thaliana, vernalization induces epigenetic silencing of the floral repressor gene FLOWERING LOCUS C (FLC). The repressive epigenetic mark trimethylation of lysine 27 on histone H3 proteins (H3K27me3) is a critical contributor to the epigenetic silencing of FLC. Interestingly, the deposited H3K27me3 in the FLC locus can be erased by short-term high-temperature treatment. This is referred to as devernalization. In this study, we identified a novel chemical compound, 4-Isoxazolecarboxylic acid, 3,5-dimethyl-2-(4-fluorophenyl)-4-isoxazole carboxylic acid 1-methyl-2-oxoethyl ester named as DEVERNALIZER01 (DVR01), which induces devernalization in Arabidopsis seedlings, by an FLC-luciferase reporter-based high-throughput screening assay. DVR01 decreased the amount of H3K27me3 in the FLC locus in vernalized plants, resulting in the upregulation of FLC in the whole plant, including the vasculature and meristem, where FLC represses floral induction genes. We also showed that a 2-week treatment with DVR01 reverted plants with a vernalized status back to a fully non-vernalized status. Collectively, this study provides a novel structure of DVR01, which modulates devernalization via demethylation of H3K27me3 in the FLC locus. Vernalization is the promotion of flowering after prolonged exposure to cold. In Arabidopsis thaliana , vernalization induces epigenetic silencing of the floral repressor gene FLOWERING LOCUS C ( FLC ). The repressive epigenetic mark trimethylation of lysine 27 on histone H3 proteins (H3K27me3) is a critical contributor to the epigenetic silencing of FLC . Interestingly, the deposited H3K27me3 in the FLC locus can be erased by short-term high-temperature treatment. This is referred to as devernalization. In this study, we identified a novel chemical compound, 4-Isoxazolecarboxylic acid, 3,5-dimethyl-2-(4-fluorophenyl)-4-isoxazole carboxylic acid 1-methyl-2-oxoethyl ester named as DEVERNALIZER01 (DVR01), which induces devernalization in Arabidopsis seedlings, by an FLC -luciferase reporter-based high-throughput screening assay. DVR01 decreased the amount of H3K27me3 in the FLC locus in vernalized plants, resulting in the upregulation of FLC in the whole plant, including the vasculature and meristem, where FLC represses floral induction genes. We also showed that a 2-week treatment with DVR01 reverted plants with a vernalized status back to a fully non-vernalized status. Collectively, this study provides a novel structure of DVR01, which modulates devernalization via demethylation of H3K27me3 in the FLC locus. |
| Author | Morisaki, Yukaho Gan, Eng-Seng Ito, Toshiro Shirakawa, Makoto Sato, Ayato |
| AuthorAffiliation | 1 Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology , Ikoma , Japan 2 Temasek Life Sciences Laboratory, National University of Singapore , Singapore , Singapore 3 Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University , Nagoya , Japan |
| AuthorAffiliation_xml | – name: 1 Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology , Ikoma , Japan – name: 2 Temasek Life Sciences Laboratory, National University of Singapore , Singapore , Singapore – name: 3 Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University , Nagoya , Japan |
| Author_xml | – sequence: 1 givenname: Makoto surname: Shirakawa fullname: Shirakawa, Makoto organization: Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Japan – sequence: 2 givenname: Yukaho surname: Morisaki fullname: Morisaki, Yukaho organization: Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Japan – sequence: 3 givenname: Eng-Seng surname: Gan fullname: Gan, Eng-Seng organization: Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore – sequence: 4 givenname: Ayato surname: Sato fullname: Sato, Ayato organization: Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan – sequence: 5 givenname: Toshiro surname: Ito fullname: Ito, Toshiro organization: Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33613612$$D View this record in MEDLINE/PubMed |
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| Keywords | devernalization Arabidopsis high-throughput screening vernalization FLC chemical screening epigenetics H3K27me3 |
| Language | English |
| License | Copyright © 2021 Shirakawa, Morisaki, Gan, Sato and Ito. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Maria Helena S. Goldman, University of São Paulo, Brazil Reviewed by: Ryo Fujimoto, Kobe University, Japan; Chris Helliwell, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia ORCID: Eng-Seng Gan, orcid.org/0000-0001-9753-4524 This article was submitted to Plant Development and EvoDevo, a section of the journal Frontiers in Plant Science |
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| Snippet | Vernalization is the promotion of flowering after prolonged exposure to cold. In
, vernalization induces epigenetic silencing of the floral repressor gene
(
).... Vernalization is the promotion of flowering after prolonged exposure to cold. In Arabidopsis thaliana , vernalization induces epigenetic silencing of the... Vernalization is the promotion of flowering after prolonged exposure to cold. In Arabidopsis thaliana, vernalization induces epigenetic silencing of the floral... |
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| StartPage | 634068 |
| SubjectTerms | chemical screening devernalization epigenetics FLC H3K27me3 Plant Science vernalization |
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| Title | Identification of a Devernalization Inducer by Chemical Screening Approaches in Arabidopsis thaliana |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/33613612 https://search.proquest.com/docview/2492278117 https://pubmed.ncbi.nlm.nih.gov/PMC7890032 https://doaj.org/article/ed7c969eb4a3463b88ab784dd9164fae |
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