Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas

• Published in: Cell research Vol. 26; no. 3; pp. 304 - 319
• Main Authors: Hou, Yu, Guo, Huahu, Cao, Chen, Li, Xianlong, Hu, Boqiang, Zhu, Ping, Wu, Xinglong, Wen, Lu, Tang, Fuchou, Huang, Yanyi, Peng, Jirun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2016; Nature Publishing Group
• Subjects: 631/208/176/1988; 631/61/212/2019; 631/61/514/1948; 692/4028/67/1504/1610/4029; Animals; Biomedical and Life Sciences; Carcinoma, Hepatocellular - genetics; Cell Biology; Deoxyribonucleic acid; DNA; DNA Copy Number Variations; DNA Methylation; DNA甲基化; Epigenesis, Genetic; Epigenomics - methods; Gene Expression Profiling - methods; Genomics; Genomics - methods; Hep G2 Cells; Heterogeneity; Humans; Life Sciences; Liver Neoplasms - genetics; Male; Mice; Middle Aged; Original; original-article; Sequence Analysis, DNA; Single-Cell Analysis; Subpopulations; Transcriptome; 单细胞; 哺乳动物细胞; 异质性; 测序方法; 肝细胞癌; 表观遗传学; 转录组; DNA methylome; HCC; CNV; scTrio-seq; transcriptome

Single-ceU genome, DNA methylome, and transcriptome sequencing methods have been separately developed. However, to accurately analyze the mechanism by which transcriptome, genome and DNA methylome regulate each other, these omic methods need to be performed in the same single cell. Here we demonstra...