A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro

• Published in: Cells (Basel, Switzerland) Vol. 9; no. 8; p. 1900
• Main Authors: Keller, Florian, Bruch, Roman, Schneider, Richard, Meier-Hubberten, Julia, Hafner, Mathias, Rudolf, Rüdiger
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.08.2020; MDPI
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Antibodies; Apoptosis; Autophagy; Biopsy; Cell culture; Coculture Techniques; Collagen; Colon cancer; Colonic Neoplasms - metabolism; Colonic Neoplasms - pathology; Colorectal cancer; Confocal microscopy; DNA microarrays; Fibroblasts; Fibroblasts - metabolism; Glucose - metabolism; Glycolysis; Hexokinase; HT29 Cells; Humans; Hypotheses; Immunofluorescence; L-Lactate dehydrogenase; Lactic acid; LC3; MCT4; Medical research; Membrane Potential, Mitochondrial; Metabolism; Metabolites; Microtubule-associated proteins; Microtubule-Associated Proteins - metabolism; Mitochondria; Mitochondria - metabolism; Monocarboxylic Acid Transporters - metabolism; Muscle Proteins - metabolism; optical tissue clearing; p53 Protein; Physiological aspects; reverse Warburg effect; Spheroids; Spheroids, Cellular - metabolism; Stromal cells; Stromal Cells - metabolism; Translocase; Tumor Microenvironment; Tumors; Ubiquitin; Warburg Effect, Oncologic; Germany; optical tissue clearing; MCT4; fibroblasts; reverse Warburg effect; LC3; mitochondria

Most tumors consume large amounts of glucose. Concepts to explain the mechanisms that mediate the achievement of this metabolic need have proposed a switch of the tumor mass to aerobic glycolysis. Depending on whether primarily tumor or stroma cells undergo such a commutation, the terms 'Warbur...