Insulin-like growth factor I in cats: validation of an enzyme-linked immunosorbent assay and determination of biologic variation

• Published in: Veterinary clinical pathology Vol. 44; no. 4; pp. 542 - 551
• Main Authors: Strage, Emma M., Theodorsson, Elvar, Ström Holst, Bodil, Lilliehöök, Inger, Lewitt, Moira S.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.12.2015; Wiley Subscription Services, Inc
• Subjects: Acromegaly - blood; Acromegaly - metabolism; Acromegaly - veterinary; Animals; Annan veterinärmedicin; Cat Diseases - blood; Cat Diseases - metabolism; Cats; Diabetes Mellitus - blood; Diabetes Mellitus - metabolism; Diabetes Mellitus - veterinary; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Growth hormone disorders; Humans; Insulin-Like Growth Factor Binding Proteins - blood; Insulin-Like Growth Factor Binding Proteins - metabolism; Insulin-Like Growth Factor I - metabolism; insulin-like growth factor-binding proteins; Other Veterinary Science; Reference Values; Reproducibility of Results; size-exclusion chromatography; size-exclusion chromatography; Growth hormone disorders; insulin-like growth factor-binding proteins

Background Insulin‐like growth factor I (IGF‐I) measurements are used in veterinary medicine for diagnosing growth hormone disorders. IGF‐I assays are subject to interference by IGF‐binding proteins (IGFBP) which may not be efficiently removed by standard extraction methods. Adding excess IGF‐II dur...