rho‐mediated protein tyrosine phosphorylation in lysophosphatidic‐acid‐induced tumor‐cell invasion

• Published in: International journal of cancer Vol. 65; no. 5; pp. 627 - 632
• Main Authors: Imamura, Fumio, Shinkai, Kiyoko, Mukai, Mutsuko, Yoshioka, Kiyoko, Komagome, Rika, Iwasaki, Teruo, Akedo, Hitoshi
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 01.03.1996; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; Cell Adhesion Molecules - metabolism; Cytoskeletal Proteins - metabolism; Enzyme Inhibitors - pharmacology; Focal Adhesion Kinase 1; Focal Adhesion Protein-Tyrosine Kinases; Gastroenterology. Liver. Pancreas. Abdomen; Genistein; GTP-Binding Proteins - metabolism; Isoflavones - pharmacology; Liver Neoplasms, Experimental - metabolism; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Lysophospholipids - pharmacology; Medical sciences; Neoplasm Invasiveness; Paxillin; Phosphoproteins - metabolism; Phosphorylation; Phosphotyrosine - metabolism; Protein-Tyrosine Kinases - antagonists & inhibitors; Protein-Tyrosine Kinases - metabolism; Rats; rho GTP-Binding Proteins; Signal Transduction; Tumor Cells, Cultured; Tumors; Tyrosine; Phosphorylation; Carcinoma; Pathophysiology; Rat; Liver; Rodentia; Hepatic disease; Exploration; Metastasis; Malignant tumor; In vitro; Invasion; Vertebrata; Mammalia; Malignant effusion; Animal; Digestive diseases; Ascites; Tumor cell; Peritoneum

Rat ascites hepatoma cells (MMI) invade a mesothelial cell monolayer in vitro in assay medium containing serum, but not in serum‐free medium. Serum could be completely replaced by I‐oleoyl lysophosphatidic acid (LPA) in inducing invasion. LPA‐induced invasion was inhibited by genistein, a tyrosine‐k...