Ribonuclease activity is a common property of Arabidopsis CCCH-containing zinc-finger proteins

• Published in: FEBS letters Vol. 582; no. 17; pp. 2577 - 2582
• Main Authors: Addepalli, Balasubrahmanyam, Hunt, Arthur G.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 23.07.2008
• Subjects: Amino Acid Motifs; Arabidopsis; Arabidopsis - enzymology; Arabidopsis Proteins - classification; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; maltose binding protein; MBP; PCR; Polyadenylation; polymerase chain reaction; reverse transcription/polymerase chain reaction; Ribonuclease; Ribonucleases - classification; Ribonucleases - genetics; Ribonucleases - metabolism; RNA binding; RT-PCR; SDS; sodium dodecyl sulfate; Zinc Fingers; Zinc-finger protein; SDS; Zinc-finger protein; RT-PCR; Polyadenylation; MBP; Ribonuclease; PCR; RNA binding
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2008.06.029

The CCCH class of zinc fingers occurs in a large number of Arabidopsis proteins. Previous studies revealed that one such protein is a nuclease, the activity of which is attributable to one of the CCCH motifs. To examine whether nuclease activity is a more general characteristic of CCCH zinc finger containing proteins, five other such Arabidopsis proteins were assayed for a similar activity. The results indicate that all of these proteins possess nuclease activity. Thus, nuclease activity may be a common characteristic of Arabidopsis CCCH-containing proteins.