Intestinal Microbial Ecology in Premature Infants Assessed with Non–Culture-Based Techniques

• Published in: The Journal of pediatrics Vol. 156; no. 1; pp. 20 - 25
• Main Authors: Mshvildadze, Maka, Neu, Josef, Shuster, Jonathan, Theriaque, Douglas, Li, Nan, Mai, Volker
• Format: Journal Article
• Language: English
• Published: Maryland Heights, MO Mosby, Inc 2010; Elsevier
• Subjects: Biological and medical sciences; DNA, Bacterial - analysis; Enterocolitis, Necrotizing - microbiology; Feces - microbiology; Female; General aspects; Humans; Infant, Newborn; Infant, Premature; Infant, Premature, Diseases - microbiology; Klebsiella; Male; Meconium - microbiology; Medical sciences; Pediatrics; Polymerase Chain Reaction - methods; RNA, Ribosomal, 16S; OTU; GI; rRNA; SDI; NEC; DGGE; PCR; Simpson diversity index; Denaturing gradient gel electrophoresis; Gastrointestinal; Polymerase chain reaction; Ribosomal RNA; Necrotizing entercolitis; Operational taxonomic unit; Human; Premature; Pediatrics; Digestive system; Gut; Infant; Ecology; Cultural aspect; Technique; Culture

To use high throughput techniques to analyze intestinal microbial ecology in premature neonates, who are highly susceptible to perturbations of the luminal environment associated with necrotizing enterocolitis (NEC) and late-onset sepsis. With non–culture-based techniques, we evaluated intestinal mi...