The light-sensitive dimerizer zapalog reveals distinct modes of immobilization for axonal mitochondria

• Published in: Nature cell biology Vol. 21; no. 6; pp. 768 - 777
• Main Authors: Gutnick, Amos, Banghart, Matthew R., West, Emma R., Schwarz, Thomas L.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.06.2019; Nature Publishing Group
• Subjects: 13; 13/106; 14; 14/19; 14/35; 38; 38/1; 631/57/343/2281; 631/80/128/2343; 631/80/642/333; Actin; Actins - antagonists & inhibitors; Analysis; Animals; Axons - chemistry; Axons - metabolism; Axons - radiation effects; Biomedical and Life Sciences; Bridged Bicyclo Compounds, Heterocyclic - pharmacology; Cancer Research; Cell Biology; Cells; Chlorocebus aethiops; COS Cells; Developmental Biology; Dihydrofolate reductase; Dimerization; Domains; Immobilization; Kinesin; Kinesin - chemistry; Life Sciences; Light; Microtubules - genetics; Microtubules - radiation effects; Mitochondria; Mitochondria - chemistry; Mitochondria - genetics; Mitochondria - radiation effects; Motility; Motors; Neurons - chemistry; Neurons - radiation effects; Photolysis; Polymerization; Polymerization - drug effects; Protein Domains - genetics; Protein Domains - radiation effects; Protein Multimerization - genetics; Protein Multimerization - radiation effects; Protein-protein interactions; Restoration; Stem Cells; Synapses - chemistry; Synapses - genetics; Synapses - radiation effects; Tacrolimus Binding Proteins - chemistry; Tacrolimus Binding Proteins - genetics; Tacrolimus-binding protein; technical-report; Thiazolidines - pharmacology; Vesicular Glutamate Transport Protein 1 - genetics

Controlling cellular processes with light can help elucidate their underlying mechanisms. Here we present zapalog, a small-molecule dimerizer that undergoes photolysis when exposed to blue light. Zapalog dimerizes any two proteins tagged with the FKBP and DHFR domains until exposure to light causes...