Purification of Antioxidant Peptides by High Resolution Mass Spectrometry from Simulated Gastrointestinal Digestion Hydrolysates of Alaska Pollock (Theragra chalcogramma) Skin Collagen

• Published in: Marine drugs Vol. 14; no. 10; p. 186
• Main Authors: Sun, Liping, Chang, Weidan, Ma, Qingyu, Zhuang, Yongliang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.10.2016; MDPI
• Subjects: Alaska pollock skin collagen; Amino acids; Amino Acids - chemistry; Animals; antioxidant peptide; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Benzothiazoles - chemistry; Collagen - chemistry; de novo software; Digestive system; Fish Proteins; Free Radical Scavengers - chemistry; Free Radical Scavengers - pharmacology; Gadiformes - metabolism; Gastrointestinal tract; Gastrointestinal Tract - chemistry; Hydrolysis; Hydroxyl radicals; Liquid chromatography; Marine; Mass spectrometry; Molecular Weight; peptide purification; Peptides; Peptides - chemistry; Peptides - pharmacology; Protein Hydrolysates - chemistry; Protein Hydrolysates - pharmacology; simulated gastrointestinal digestions; Skin - chemistry; Spectrometry, Mass, Electrospray Ionization; Sulfonic Acids - chemistry; Theragra chalcogramma; UniProt of MaxQuant; INE, USA, Alaska; antioxidant peptide; peptide purification; Alaska pollock skin collagen; de novo software; UniProt of MaxQuant; simulated gastrointestinal digestions

In this study, the stable collagen hydrolysate was prepared by alcalase hydrolysis and twice simulated gastrointestinal digestion from Alaska pollock skin. The characteristics of hydrolysates and antioxidant activities in vitro, including 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radic...