Sequential phosphoproteomics and N-glycoproteomics of plasma-derived extracellular vesicles

• Published in: Nature protocols Vol. 15; no. 1; pp. 161 - 180
• Main Authors: Andaluz Aguilar, Hillary, Iliuk, Anton B., Chen, I-Hsuan, Tao, W. Andy
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.01.2020; Nature Publishing Group
• Subjects: 631/1647/296; 631/80/313/1481; 631/92/458/1524; 631/92/458/1733; 631/92/475; Analytical Chemistry; Antibiotics; Biological Techniques; Biomarkers; Biomedical and Life Sciences; Blood plasma; Cell organelles; Cell signaling; Composition; Computational Biology/Bioinformatics; Extracellular vesicles; Extracellular Vesicles - metabolism; Glycopeptides; Glycoproteins; Glycoproteins - metabolism; Glycosylation; Humans; Identification and classification; Life Sciences; Mass spectrometry; Mass spectroscopy; Metabolites; Methods; Microarrays; Modularity; Organic Chemistry; Phosphoproteins; Phosphoproteins - metabolism; Phosphorylation; Plasma - cytology; Post-translation; Protein Processing, Post-Translational; Proteins; Proteomics; Proteomics - methods; Protocol; Uniqueness; Vesicles; Workflow; United States

Extracellular vesicles (EVs) are increasingly being recognized as important vehicles for intercellular communication and as promising sources for biomarker discovery. Because the state of protein post-translational modifications (PTMs) such as phosphorylation and glycosylation can be a key determina...