Isolation of high molecular weight and humic acid-free metagenomic DNA from lignocellulose-rich samples compatible for direct fosmid cloning

• Published in: Applied microbiology and biotechnology Vol. 102; no. 14; pp. 6207 - 6219
• Main Authors: Sar, Abhijit, Pal, Srikanta, Dam, Bomba
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2018; Springer; Springer Nature B.V
• Subjects: Acids; Aluminum; Aluminum sulfate; Bioactive compounds; Biomass; Biomedical and Life Sciences; Biotechnology; Chloroform; Cloning; composts; Deoxyribonucleic acid; DNA; DNA libraries; Endopeptidase K; Enzymes; Flocculation; Forest soils; gardens; genes; Genomics; horticultural soils; Humic acids; Humic substances; lakes; Life Sciences; Lignocellulose; Lysis; Lysozyme; Metagenomics; Methods; Methods and Protocols; microbial biomass; Microbial Genetics and Genomics; Microbiological research; Microbiology; Microorganisms; Molecular chains; Molecular weight; Nucleic acid isolation; Organic chemistry; peptidase K; Phenols; Physiological aspects; Polyethylene glycol; polymerase chain reaction; Proteinase; protocols; Purity; Renewable energy; Rice straw; Screening; Sodium chloride; Straw; wood; High molecular weight (HMW) DNA; Lignocellulose; Flocculation; Industrial enzymes; Functional metagenomics; Bioenergy; Humic acid

Activity-based screening of metagenomic DNA libraries is a promising approach to fish out genes encoding novel bioactive compounds/enzymes of industrial importance. The starting point of such functional screening in fosmid vectors is isolation of high molecular weight (HMW) DNA of sufficient purity...