Label-Free Detection of Single-Base Mismatches in DNA by Surface-Enhanced Raman Spectroscopy

Singles only: DNA sequences can be induced to spontaneously adsorb to the surfaces of Ag colloids through their nucleotide side chains (see picture). The SERS spectra of these nonspecifically bound strands are sufficiently reproducible that they can be used to identify single‐base mismatches in shor...

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Published inAngewandte Chemie (International ed.) Vol. 50; no. 39; pp. 9058 - 9061
Main Authors Papadopoulou, Evanthia, Bell, Steven E. J.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 19.09.2011
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
Base Pair Mismatch
Base Sequence
Colloids - chemistry
Communications
DNA
DNA - analysis
nanomaterials
nucleotides
RNA
SERS
Silver - chemistry
Spectrum Analysis, Raman - methods
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Summary:Singles only: DNA sequences can be induced to spontaneously adsorb to the surfaces of Ag colloids through their nucleotide side chains (see picture). The SERS spectra of these nonspecifically bound strands are sufficiently reproducible that they can be used to identify single‐base mismatches in short (25‐mer and 23‐mer) strands. Subtracting the spectra of different DNA sequences results in difference spectra that contain features corresponding to the exchanged nucleotides.
Bibliography:Queen's University
ark:/67375/WNG-F3L2NWLN-R
ArticleID:ANIE201102776
E. P. thanks Queen's University, Belfast for the award of a McClay Trust Studentship.
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Supporting information for this article is available on the WWW under http://dx.doi.org/10.1002/anie.201102776.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201102776