尼罗罗非鱼γ-干扰素基因的克隆、结构分析及组织表达
γ-干扰素（interferon,IFN）是调控天然免疫与细胞免疫的一种重要细胞因子,在抵抗病毒入侵和细菌感染中发挥重要作用。该研究从尼罗罗非鱼（Oreochromis niloticus）脾脏组织中扩增到γ-干扰素基因的c DNA,大小为621 bp,编码206个氨基酸。生物信息学分析表明,尼罗罗非鱼γ-干扰素分子（On IFNγ）具有信号肽、IFN特征序列及核定位信号。预测的三级结构与人类IFNγ晶体结构最相似。q PCR检测显示,IFNγ在正常鱼体内的脾脏中表达量最高,其次是肠、鳃和肾脏。感染无乳链球菌（Streptococcus agalactiae）后,On IFNγ在脾脏、肾和鳃中...
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| Published in | 南方水产科学 Vol. 13; no. 1; pp. 85 - 93 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东 广州 510380
2017
上海海洋大学水产与生命学院,上海201306%中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东 广州 510380 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0780 |
| DOI | 10.3969/j.issn.2095-0780.2017.01.011 |
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| Abstract | γ-干扰素（interferon,IFN）是调控天然免疫与细胞免疫的一种重要细胞因子,在抵抗病毒入侵和细菌感染中发挥重要作用。该研究从尼罗罗非鱼（Oreochromis niloticus）脾脏组织中扩增到γ-干扰素基因的c DNA,大小为621 bp,编码206个氨基酸。生物信息学分析表明,尼罗罗非鱼γ-干扰素分子（On IFNγ）具有信号肽、IFN特征序列及核定位信号。预测的三级结构与人类IFNγ晶体结构最相似。q PCR检测显示,IFNγ在正常鱼体内的脾脏中表达量最高,其次是肠、鳃和肾脏。感染无乳链球菌（Streptococcus agalactiae）后,On IFNγ在脾脏、肾和鳃中的表达均显著上调（P〈0.05）。On IFNγ在相关免疫器官中的高表达、受感染后的表达上调,说明其在罗非鱼免疫防御中可能具有重要作用。该研究为进一步开展On IFNγ基因的功能研究、探讨其在抗病转基因及抗病选育中的开发应用奠定了基础。 |
|---|---|
| AbstractList | γ-干扰素（interferon,IFN）是调控天然免疫与细胞免疫的一种重要细胞因子,在抵抗病毒入侵和细菌感染中发挥重要作用。该研究从尼罗罗非鱼（Oreochromis niloticus）脾脏组织中扩增到γ-干扰素基因的c DNA,大小为621 bp,编码206个氨基酸。生物信息学分析表明,尼罗罗非鱼γ-干扰素分子（On IFNγ）具有信号肽、IFN特征序列及核定位信号。预测的三级结构与人类IFNγ晶体结构最相似。q PCR检测显示,IFNγ在正常鱼体内的脾脏中表达量最高,其次是肠、鳃和肾脏。感染无乳链球菌（Streptococcus agalactiae）后,On IFNγ在脾脏、肾和鳃中的表达均显著上调（P〈0.05）。On IFNγ在相关免疫器官中的高表达、受感染后的表达上调,说明其在罗非鱼免疫防御中可能具有重要作用。该研究为进一步开展On IFNγ基因的功能研究、探讨其在抗病转基因及抗病选育中的开发应用奠定了基础。 S917.4; γ-干扰素(interferon,IFN)是调控天然免疫与细胞免疫的一种重要细胞因子,在抵抗病毒入侵和细菌感染中发挥重要作用.该研究从尼罗罗非鱼(Oreochromis niloticus)脾脏组织中扩增到γ-干扰素基因的cDNA,大小为621 bp,编码206个氨基酸.生物信息学分析表明,尼罗罗非鱼γ-干扰素分子(On IFN γ)具有信号肽、IFN特征序列及核定位信号.预测的三级结构与人类IFN γ晶体结构最相似.qPCR检测显示,IFNγ在正常鱼体内的脾脏中表达量最高,其次是肠、鳃和肾脏.感染无乳链球菌(Streptococcus agalactiae)后,On IFN γ在脾脏、肾和鳃中的表达均显著上调(P<0.05).On IFN γ在相关免疫器官中的高表达、受感染后的表达上调,说明其在罗非鱼免疫防御中可能具有重要作用.该研究为进一步开展On IFN γ基因的功能研究、探讨其在抗病转基因及抗病选育中的开发应用奠定了基础. |
| Abstract_FL | Interferon-γ is a cytokine crucial for the regulation of both natural and cellular immunity,playing an important role in defending against virus invasion and bacterial infection.In this study,the cDNA of interferon-γ genes was amplified from the spleen tissues of Nile tilapia;this cDNA had an open reading frame of 621 bp,encodeing 206 amino acids.Bioinformatics analysis shows that the interferon-γ molecule of Nile tilapia contains one signal peptide and one nuclear localization signal.Its tertiary structure was similar with the crystal structure of human interferon-γ.Real-time fluorescent quantitative polymerase chain reaction(PCR)shows that the expression level of interferon-γ gene was the highest in the spleen of normal Nile tilapia,followed by the intestines,gills and kidneys(P<0.05).Both the high expression of interferon-γ in immune-associated tissues and its upregulated expression after GBS infection reveal the significant role of interferon-γ in the immune defense of Nile tilapia.This study lays foundation for further research on the function of interferon-γ gene in Nile tilapia as well as its development and application in transgenesis and selective breeding for disease resistance. |
| Author | 余嘉欣 吴芳 孙成飞 董浚键 田园园 叶星 |
| AuthorAffiliation | 中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 上海海洋大学水产与生命学院,上海201306 |
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| DocumentTitleAlternate | Cloning,structure analysis and expression of interferon gamma （IFN-γ） gene in Oreochromis niloticus |
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| Keywords | 生物信息学 Oreochromis niloticus interferon-γ 组织分布 bioinformatics 序列分析 structure analysis 尼罗罗非鱼 sequence analysis γ-干扰素 结构分析 tissue distribution |
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| Notes | Interferon-γ is a cytokine crucial for the regulation of both natural and cellular immunity,playing an important role in defending against virus invasion and bacterial infection. In this study,the c DNA of interferon-γ genes was amplified from the spleen tissues of Nile tilapia; this c DNA had an open reading frame of 621 bp,encodeing 206 amino acids. Bioinformatics analysis shows that the interferon-γ molecule of Nile tilapia contains one signal peptide and one nuclear localization signal. Its tertiary structure was similar with the crystal structure of human interferon-γ. Real-time fluorescent quantitative polymerase chain reaction（ PCR） shows that the expression level of interferon-γ gene was the highest in the spleen of normal Nile tilapia,followed by the intestines,gills and kidneys（ P 〈0. 05）. Both the high expression of interferon-γ in immune-associated tissues and its upregulated expression after GBS infection reveal the significant role of interferon-γ in the immune defense of Nile tilapia. This stud |
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| PublicationTitle | 南方水产科学 |
| PublicationTitleAlternate | South China Fisheries Science |
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| PublicationYear | 2017 |
| Publisher | 中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东 广州 510380 上海海洋大学水产与生命学院,上海201306%中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东 广州 510380 |
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| Snippet | γ-干扰素（interferon,IFN）是调控天然免疫与细胞免疫的一种重要细胞因子,在抵抗病毒入侵和细菌感染中发挥重要作用。该研究从尼罗罗非鱼（Oreochromis niloticus）脾脏组织中扩增到γ-干扰素基因的c DNA,大小为621... S917.4; γ-干扰素(interferon,IFN)是调控天然免疫与细胞免疫的一种重要细胞因子,在抵抗病毒入侵和细菌感染中发挥重要作用.该研究从尼罗罗非鱼(Oreochromis niloticus)脾脏组织中扩增到γ-干扰素基因的cDNA,大小为621... |
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| SubjectTerms | γ-干扰素 尼罗罗非鱼 序列分析 生物信息学 组织分布 结构分析 |
| Title | 尼罗罗非鱼γ-干扰素基因的克隆、结构分析及组织表达 |
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