Evaluation of efficacy, biodistribution and safety of antibiotic-free plasmid encoding somatostatin genes delivered by attenuated Salmonella enterica serovar Choleraesuis

• Published in: Vaccine Vol. 32; no. 12; pp. 1368 - 1374
• Main Authors: Liang, Aixin, Riaz, Hasan, Dong, Fangxiao, Luo, Xuan, Yu, Xue, Han, Yanguo, Chong, Zhenlu, Han, Li, Guo, Aizhen, Yang, Liguo
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 10.03.2014; Elsevier
• Subjects: Administration, Oral; Allergy and Immunology; Animals; antibiotic resistance; antibodies; Applied microbiology; Aspartate-Semialdehyde Dehydrogenase - genetics; bacteria; Bacteriology; Biological and medical sciences; brain; DNA vaccine; domestic animals; Female; Fundamental and applied biological sciences. Psychology; genes; Genes, Reporter; Immunity, Humoral; immunoglobulin A; immunoglobulin G; Immunoglobulin G - blood; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; mutants; plasmids; Plasmids - genetics; Plasmids - immunology; Plasmids - pharmacokinetics; recombinant vaccines; risk; Safety; Salmonella; Salmonella choleraesuis Choleraesuis; Salmonella enterica; Salmonella enterica - genetics; Salmonella enterica - immunology; Somatostatin; Somatostatin - genetics; Somatostatin - immunology; Tissue Distribution; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, DNA - genetics; Vaccines, DNA - immunology; DNA vaccine; Salmonella; Mice; Somatostatin; Safety; Peptide hormone; Rodentia; Genetic vaccine; Neuropeptide; Plasmid; Vertebrata; Antibiotic; Mammalia; Gene; Mouse; Efficiency; Salmonella choleraesuis; Bacteria; Enterobacteriaceae

•Asd-based balanced-lethal system for DNA vaccine was evaluated in this paper.•The C501(pVGS/2SS-asd) vaccine could induce systemic and mucosal immune responses.•The C501 (pVGS/2SS-asd) vaccine is safe and stable for clinical use in large animals.•The risk of gene integration into the host cellular...