Linarin down-regulates phagocytosis, pro-inflammatory cytokine production, and activation marker expression in RAW264.7 macrophages

• Published in: Food science and biotechnology Vol. 25; no. 5; pp. 1437 - 1442
• Main Authors: Kim, Bomi, Lee, Jong Hun, Seo, Myung-Ji, Eom, Seok Hyun, Kim, Wooki
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society of Food Science and Technology 01.10.2016; Springer Nature B.V; 한국식품과학회
• Subjects: anti-inflammatory activity; Anticancer properties; Antigen presentation; Antigens; antineoplastic activity; Antioxidants; Beads; Bioactive compounds; Biocompatibility; CD80 antigen; Chemistry; Chemistry and Materials Science; Chrysanthemum; Chrysanthemum indicum; coculture; Cytokines; Cytotoxicity; Dendranthema; dose response; Flavone glycosides; Flavonoids; Fluorescence; Food Science; Glycosides; IL-1β; Inflammation; Interleukin 6; interleukin-1beta; Lipopolysaccharides; Macrophages; Markers; Nitric oxide; Nutrition; Phagocytes; Phagocytosis; Plant extracts; secretion; Surface markers; Toxicity; 식품과학; cytokine; phagocytosis; flavonoid; inflammation; antigen-presentation
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-016-0223-3

Plant-extracted flavonoid glycosides have been reported to be bioactive compounds with pleiotropic functions, including antioxidant, anti-inflammatory, and anti-cancer effects. This study investigated the anti-inflammatory role of linarin (acacetin-7-rutinoside, which is found in Chrysanthemum indicum (Gam-Guk) and Dendranthema zawadskii (Gu-Jul-Cho)), on lipopolysaccharide-stimulated RAW264.7 macrophages. Linarin treatments exhibited no cytotoxicity up to a concentration of 30 μM, as assessed by MTT assay. The production of nitric oxide, an inflammatory mediator, was decreased by addition of linarin. The secretion of pro-inflammatory cytokines, interleukin-1β and interleukin-6, was significantly decreased in a dose-dependent manner. Linarin also decreased the phagocytic ability of macrophages following co-culture with fluorescent beads. In addition, expression levels of antigenpresenting surface markers, MHC II and CD80, were suppressed by linarin. Taken together, these results indicate that the flavonoid glycoside linarin has an anti-inflammatory effect, in part through the suppression of phagocytosis, cytokine production, and antigen presentation in macrophages.