Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus

• Published in: Journal of virological methods Vol. 258; pp. 41 - 48
• Main Authors: Shirato, Kazuya, Semba, Shohei, El-Kafrawy, Sherif A., Hassan, Ahmed M., Tolah, Ahmed M., Takayama, Ikuyo, Kageyama, Tsutomu, Notomi, Tsugunori, Kamitani, Wataru, Matsuyama, Shutoku, Azhar, Esam Ibraheem
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2018; The Authors. Published by Elsevier B.V
• Subjects: Animals; camels; Camelus; Coronavirus Infections - diagnosis; Coronavirus Infections - veterinary; cross reaction; diagnostic techniques; DNA; DNA Primers - genetics; Fluorescence; Humans; magnesium; MERS coronavirus; Middle East respiratory syndrome; Middle East respiratory syndrome coronavirus; Middle East Respiratory Syndrome Coronavirus - genetics; Middle East Respiratory Syndrome Coronavirus - isolation & purification; Molecular Diagnostic Techniques - methods; monitoring; Nucleic Acid Amplification Techniques - methods; nucleocapsid; oligodeoxyribonucleotides; Oligonucleotide Probes - genetics; patients; quantitative polymerase chain reaction; Quenching probe; rapid methods; reverse transcriptase polymerase chain reaction; reverse transcription loop-mediated isothermal amplification; RNA; RT-LAMP; Saudi Arabia; Sensitivity and Specificity; transcription (genetics); turbidity; viruses; Saudi Arabia; HBoV; PBS; ORF; HCoV; Quenching probe; BIP; CoV; upE; FIP; TCID50; ATCC; Middle East respiratory syndrome; N; RT-LAMP; MPV; ADV; FFU; RSV; QProbe or QP; PIV; MERS coronavirus; MERS; PFU

•Fluorescent RT-LAMP assays using quenching probes for MERS-CoV were developed.•Quenching probe (QProbe) can solve the problem in turbidity monitoring mechanism.•Only primer-derived signal can be monitored specifically by QProbes.•Two primer sets were developed to enable to confirm MERS case by RT-L...