Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus - Shirato, Kazuya; Semba, Shohei; El-Kafrawy, Sherif A.; Hassan, Ahmed M.; Tolah, Ahmed M.; Takayama, Ikuyo; Kageyama, Tsutomu; Notomi, Tsugunori; Kamitani, Wataru; Matsuyama, Shutoku; Azhar, Esam Ibraheem (Journal of virological methods, 2018)
Keywords: Animals; camels; Camelus; Coronavirus Infections - diagnosis; Coronavirus Infections - veterinary; cross reaction; diagnostic techniques; DNA; DNA Primers - genetics; Fluorescence; Humans; magnesium; MERS coronavirus; Middle East respiratory syndrome; Middle East respiratory syndrome coronavirus; Middle East Respiratory Syndrome Coronavirus - genetics; Middle East Respiratory Syndrome Coronavirus - isolation & purification; Molecular Diagnostic Techniques - methods; monitoring; Nucleic Acid Amplification Techniques - methods; nucleocapsid; oligodeoxyribonucleotides; Oligonucleotide Probes - genetics; patients; quantitative polymerase chain reaction; Quenching probe; rapid methods; reverse transcriptase polymerase chain reaction; reverse transcription loop-mediated isothermal amplification; RNA; RT-LAMP; Saudi Arabia; Sensitivity and Specificity; transcription (genetics); turbidity; viruses; Saudi Arabia; HBoV; PBS; ORF; HCoV; Quenching probe; BIP; CoV; upE; FIP; TCID50; ATCC; Middle East respiratory syndrome; N; RT-LAMP; MPV; ADV; FFU; RSV; QProbe or QP; PIV; MERS coronavirus; MERS; PFU