Detection of human immunodeficiency virus RNAs in living cells using Spinach RNA aptamers

• Published in: Virus research Vol. 228; pp. 141 - 146
• Main Authors: Burch, Brandon D., Garrido, Carolina, Margolis, David M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.01.2017
• Subjects: Aptamers; Aptamers, Nucleotide; Cell Line; Cells, Cultured; HIV - genetics; HIV Infections - diagnosis; HIV Infections - virology; HIV-RNA; Humans; Lentivirus; Microscopy, Fluorescence - methods; Retroviridae; RNA, Plant; RNA, Viral - genetics; SELEX Aptamer Technique; Spectrometry, Fluorescence - methods; Spinach; Spinacia oleracea; Spinacia oleracea - genetics; Virion; Virus Replication; HIV-RNA; Aptamers; Spinach

•Limited techniques allow in vivo RNA expression to be imaged in real time.•Better understanding the kinetics of transcriptional regulation and expression in vivo is an unmet need.•We present a novel technique that allows the real-time, single cell, visualization of HIV RNA expression in vivo in unm...