Comparison of secretome from osteoblasts derived from sclerotic versus non-sclerotic subchondral bone in OA: A pilot study

• Published in: PloS one Vol. 13; no. 3; p. e0194591
• Main Authors: Sanchez, Christelle, Mazzucchelli, Gabriel, Lambert, Cécile, Comblain, Fanny, DePauw, Edwin, Henrotin, Yves
• Format: Journal Article Web Resource
• Language: English
• Published: United States Public Library of Science 2018; Public Library of Science (PLoS)
• Subjects: Abnormalities; Adult; Aged; Aged, 80 and over; Aging; Arthritis; Biocompatibility; Biology and Life Sciences; Biomarkers; Biomarkers - analysis; Bone and Bones - cytology; Bone and Bones - metabolism; Bone and Bones - pathology; Bone matrix; Bone remodeling; Bone Remodeling - physiology; Bone turnover; Calcification, Physiologic; Cartilage; Cartilage diseases; Cell culture; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Epitopes; Extracellular Matrix Proteins - metabolism; Glycoproteins; Growth factors; Human health sciences; Humans; Immunoassay; Immunoassays; Insulin-like growth factor II; Insulin-like growth factor-binding protein 2; Insulin-like growth factor-binding protein 3; Insulin-like growth factor-binding protein 5; Insulin-like growth factor-binding protein 6; Laboratories; Macrophage colony-stimulating factor; Male; Mass spectrometry; Medicine and Health Sciences; Metabolism; Middle Aged; Mineralization; mRNA; Osteoarthritis; Osteoarthritis, Knee - diagnosis; Osteoarthritis, Knee - pathology; Osteoblasts; Osteoblasts - metabolism; Osteonectin; Osteosclerosis - pathology; Patients; Penicillin; Pilot Projects; Polymerase chain reaction; Proteins; Proteoglycans - metabolism; Proteomics; Rheumatology; Rhumatologie; RNA, Messenger - metabolism; Sciences de la santé humaine; Scientific imaging; Sclerosis; Secretome; Subchondral bone; Thrombospondin; Tissue inhibitor of metalloproteinase 1; Tissues; Belgium

Osteoarthritis (OA) is characterized by cartilage degradation but also by other joint tissues modifications like subchondral bone sclerosis. In this study, we used a proteomic approach to compare secretome of osteoblast isolated from sclerotic (SC) or non sclerotic (NSC) area of OA subchondral bone....