HIV LTR-Driven Antisense RNA by Itself Has Regulatory Function and May Curtail Virus Reactivation From Latency

• Published in: Frontiers in microbiology Vol. 9; p. 1066
• Main Authors: Kobayashi-Ishihara, Mie, Terahara, Kazutaka, Martinez, Javier P., Yamagishi, Makoto, Iwabuchi, Ryutaro, Brander, Christian, Ato, Manabu, Watanabe, Toshiki, Meyerhans, Andreas, Tsunetsugu-Yokota, Yasuko
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers 25.05.2018; Frontiers Media S.A
• Subjects: HIV; Latency; Latency reversing agents; Microbiology; Reactivation; Viral antisense RNA; reactivation; HIV; latency reversing agents; latency; viral antisense RNA

Latently infected T lymphocytes are an important barrier toward eliminating a persistent HIV infection. Here we describe an HIV-based recombinant fluorescent-lentivirus referred to as "rfl-HIV" that enables to analyze sense and antisense transcription by means of fluorescence reporter gene...