Real-time RT-PCR assays for discriminating influenza B virus Yamagata and Victoria lineages

• Published in: Journal of virological methods Vol. 205; pp. 110 - 115
• Main Authors: Nakauchi, Mina, Takayama, Ikuyo, Takahashi, Hitoshi, Oba, Kunihiro, Kubo, Hideyuki, Kaida, Atsushi, Tashiro, Masato, Kageyama, Tsutomu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2014
• Subjects: hemagglutination; Humans; influenza; Influenza B virus; Influenza B virus - classification; Influenza B virus - genetics; Influenza B virus - isolation & purification; Influenza, Human - virology; monitoring; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction - methods; Real-time RT-PCR; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA; Species Specificity; viruses; Real-time RT-PCR; Influenza B virus

•We developed one step real-time RT-PCR assays to discriminate two lineages of influenza B viruses.•The developed assays were evaluated using in vitro transcribed control RNA, clinical specimens, and clinical isolates.•The assays were shown to have high sensitivity and high specificity.•The results...