Synergistic capture of Clostridium botulinum type A neurotoxin by scFv antibodies to novel epitopes

• Published in: Biotechnology and bioengineering Vol. 108; no. 10; pp. 2456 - 2467
• Main Authors: Gray, Sean A., Barr, John R., Kalb, Suzanne R., Marks, James D., Baird, Cheryl L., Cangelosi, Gerard A., Miller, Keith D., Feldhaus, Michael J.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.10.2011; Wiley; Wiley Subscription Services, Inc
• Subjects: 60 APPLIED LIFE SCIENCES; affinity reagents; ANTIBODIES; Antibodies, Bacterial - biosynthesis; Antibodies, Bacterial - chemistry; Antibodies, Bacterial - genetics; Antibody Specificity; ANTIGENS; Binding; Binding sites; Biological and medical sciences; Biotechnology; BoNT/A; Botulinum Toxins, Type A - chemistry; Botulinum Toxins, Type A - genetics; Botulinum Toxins, Type A - metabolism; CHAINS; CLEAVAGE; Cloning; CLOSTRIDIUM BOTULINUM; DETECTION; EFFICIENCY; Epitopes - chemistry; Epitopes - genetics; Epitopes - metabolism; Fundamental and applied biological sciences. Psychology; Humans; INCUBATION; Marking; MASS SPECTROSCOPY; molecular probes; MONOCLONAL ANTIBODIES; PEPTIDES; PRODUCTION; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; scFv; Single-Chain Antibodies - biosynthesis; Single-Chain Antibodies - chemistry; Single-Chain Antibodies - genetics; TOXINS; Yeast; yeast display; YEASTS; Clostridium botulinum; Yeast; Antigenic determinant; BoNT/A; Selection; Clostridiales; Metalloendopeptidases; Fv-Fragment; Synergism; Bontoxilysin; affinity reagents; Bacteria; Molecular probe; antibodies; Enzyme; Antibody; yeast display; Peptidases; Toxin; Screening; scFv; Clostridiaceae; Neurotoxin; Hydrolases; Affinity; molecular probes

A non‐immune library of human single chain fragment variable (scFv) antibodies displayed on Saccharomyces cerevisiae was screened for binding to the Clostridium botulinum neurotoxin serotype A binding domain [BoNT/A (Hc)] with the goal of identifying scFv to novel epitopes. To do this, an antibody‐m...