Optimized Standard Operating Procedures for the Analysis of Cerebrospinal Fluid Aβ42 and the Ratios of Aβ Isoforms Using Low Protein Binding Tubes

• Published in: Journal of Alzheimer's disease Vol. 53; no. 3; pp. 1121 - 1132
• Main Authors: Vanderstichele, Hugo Marcel Johan, Janelidze, Shorena, Demeyer, Leentje, Coart, Els, Stoops, Erik, Herbst, Victor, Mauroo, Kimberley, Brix, Britta, Hansson, Oskar
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.01.2016
• Subjects: Alzheimer Disease - cerebrospinal fluid; Alzheimer Disease - pathology; Amyloid beta-Peptides - cerebrospinal fluid; Brain - metabolism; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Models, Statistical; Peptide Fragments - cerebrospinal fluid; Protein Isoforms - cerebrospinal fluid; Amyloid; improvement; cerebrospinal fluid; standard operating procedure; ELISA

Background: Reduced cerebrospinal fluid (CSF) concentration of amyloid-β1-42 (Aβ1-42) reflects the presence of amyloidopathy in brains of subjects with Alzheimer’s disease (AD). Objective: To qualify the use of Aβ1-42/Aβ1-40 for improvement of standard operating procedures (SOP) for measurement of C...