Expression of inorganic phosphate/vesicular glutamate transporters (BNPI/VGLUT1 and DNPI/VGLUT2) in the cerebellum and precerebellar nuclei of the rat

• Published in: Brain research. Molecular brain research. Vol. 107; no. 1; pp. 23 - 31
• Main Authors: Hisano, Setsuji, Sawada, Kazuhiko, Kawano, Michihiro, Kanemoto, Mizuki, Xiong, Guoxiang, Mogi, Koichi, Sakata-Haga, Hiromi, Takeda, Jun, Fukui, Yoshihiro, Nogami, Haruo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 30.10.2002; Elsevier
• Subjects: Animals; Biochemistry and metabolism; Biological and medical sciences; BNPI/VGLUT1; Brain Stem - cytology; Brain Stem - metabolism; Carrier Proteins - genetics; Carrier Proteins - metabolism; Central nervous system; Cerebellum - cytology; Cerebellum - metabolism; Cerebellum DNPI/VGLUT2; Fundamental and applied biological sciences. Psychology; Gene Expression - physiology; Glutamic Acid - metabolism; Immunohistochemistry; Male; Membrane Transport Proteins; Nerve Fibers - metabolism; Nerve Fibers - ultrastructure; Neural Pathways - cytology; Neural Pathways - metabolism; Olivary Nucleus - cytology; Olivary Nucleus - metabolism; Phosphates - metabolism; Rat; Rats; Rats, Sprague-Dawley; Reticular Formation - cytology; Reticular Formation - metabolism; RNA, Messenger - metabolism; Synaptic Transmission - physiology; Vertebrates: nervous system and sense organs; Vesicular Glutamate Transport Protein 1; Vesicular Glutamate Transport Protein 2; Vesicular glutamate/inorganic phosphate transporter; Vesicular Transport Proteins; Vestibular Nuclei - cytology; Vestibular Nuclei - metabolism; BNPI/VGLUT1; Vesicular glutamate/inorganic phosphate transporter; Cerebellum DNPI/VGLUT2; Rat; Neurotransmitters, modulators, transporters and receptors; Cerebellum; Vertebrata; Mammalia; Rodentia; Central nervous system; Excitatory aminoacid; Neurotransmitter; Gene expression; Glutamate; Carrier protein; Brain (vertebrata)
• ISSN: 0169-328X; 1872-6941
• DOI: 10.1016/S0169-328X(02)00442-4

Expression of inorganic phosphate/vesicular glutamate transporters (BNPI/VGLUT1 and DNPI/VGLUT2) was studied in the cerebellum and precerebellar nuclei of rats using immunohistochemistry and in situ hybridization. DNPI/VGLUT2-stained mossy fibers were principally seen in the vermis (lobules I and VIII–X) and flocculus, whereas BNPI/VGLUT1-stained mossy fibers were localized throughout the cortex. Some vermal and floccular mossy fibers were stained for both transporters. High levels of DNPI/VGLUT2 mRNA hybridization signals were demonstrated in many neurons throughout the vestibular nuclear complex as well as the lateral reticular, external cuneate, inferior olivary and deep cerebellar nuclei. Significant BNPI/VGLUT1 mRNA signals were demonstrated in the lateral reticular nucleus and vestibular nuclear complex but not in the inferior olivary nucleus, indicating that climbing fibers have DNPI/VGLUT2 only. These results show that DNPI/VGLUT2 is expressed preferentially to vestibulo-, reticulo- and cuneocerebellar neurons, some of which also possess BNPI/VGLUT1, suggesting some differential and co-operative functions between DNPI/VGLUT2 and BNPI/VGLUT1 in the cerebellum.