MSCs‑derived exosomes attenuate ischemia-reperfusion brain injury and inhibit microglia apoptosis might via exosomal miR-26a-5p mediated suppression of CDK6

• Published in: Molecular medicine (Cambridge, Mass.) Vol. 27; no. 1; pp. 67 - 12
• Main Authors: Cheng, Chang, Chen, Xiuying, Wang, Yuhan, Cheng, Wenchao, Zuo, Xuzheng, Tang, Weiju, Huang, Wen
• Format: Journal Article
• Language: English
• Published: England BioMed Central 02.07.2021; BMC
• Subjects: Animals; Apoptosis - genetics; Biomarkers; Brain - blood supply; Brain - metabolism; Brain - pathology; CDK6; Cyclin-Dependent Kinase 6 - genetics; Disease Models, Animal; Disease Susceptibility; Exosomes; Exosomes - metabolism; Gene Expression Regulation; Ischemia–reperfusion injury; Mesenchymal Stem Cells - metabolism; Mesenchymal stromal cells; Mice; Microglia - metabolism; MicroRNAs - genetics; miR-26a-5p; Reperfusion Injury - etiology; Reperfusion Injury - metabolism; RNA Interference; Mesenchymal stromal cells; Ischemia–reperfusion injury; CDK6; Exosomes; miR-26a-5p
• ISSN: 1076-1551; 1528-3658; 1528-3658
• DOI: 10.1186/s10020-021-00324-0

This study aimed to explore the role of mesenchymal stromal cells (MSCs)-derived exosomes (MSCs-Exo) in the cerebral ischemia-reperfusion (I/R) injury. Exosomes were isolated from MSCs of adult C57BL/6J mice by the gradient centrifugation method. The expression of miR-26a-5p and CDK6 in MSCs-Exo and mice brain tissues were evaluated by qRT-PCR and western blot. miR-26a-5p mimics and miR-NC were transfected into MSCs, and exosomes were isolated from the MSCs stably expressing miR-26a-5p. Then MSCs-Exo-miR-26a-5p mimics or MSCs-Exo-miR-NC was injected into mice through the tail vein, or added into medium to stimulate BV-2 cells. Cell viability was evaluated by CCK-8 assay. Cell apoptosis was detected by flow cytometry. The apoptosis in brain tissues was evaluated by TUNEL staining assay. Bioinformatics analysis and luciferase reporter assay were performed to determine the binding relationship between miR-26a-5p and CDK6. miR-26a-5p was downregulated and CDK6 was upregulated in MSCs-Exo of MCAO-mice and OGD-induced MSCs. MSCs-Exo-miR-26a-5p mimics significantly reduced cell apoptosis of OGD-injured BV-2 cells. MSCs-Exo-miR-26a-5p mimics significantly reduced the infarct volume of MCAO-induced mice. Luciferase reporter assay revealed that CDK-6 was a target of miR-26a-5p. In addition, MSCs-Exo-miR-26a-5p mimics significantly decreased the expression of CDK6 in both OGD-induced BV-2 cells and the brain tissues of MCAO-treated mice. Our results indicated that MSCs‑Exo attenuated I/R injury in mice by inhibiting microglia apoptosis might via exosomal miR-26a-5p mediated suppression of CDK6. Our study shed light on the application of MSC-Exo as a potential therapeutic tool for cerebral I/R injury.