An Explicit Formulation Approach for the Analysis of Calcium Binding to EF-Hand Proteins Using Isothermal Titration Calorimetry

• Published in: Biophysical journal Vol. 105; no. 12; pp. 2843 - 2853
• Main Authors: Keeler, Camille, Poon, Gregory, Kuo, Ivana Y., Ehrlich, Barbara E., Hodsdon, Michael E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 17.12.2013; Biophysical Society; The Biophysical Society
• Subjects: Binding sites; Biophysics; Calcium; Calcium - metabolism; Calorimetry; Calorimetry - methods; chelating agents; computer software; Differential equations; EF Hand Motifs; equations; functional properties; Humans; Isotherms; Ligands; Mathematical models; Mathematical problems; Models, Biological; Ordinary differential equations; polycystic kidney diseases; polycystins; Protein Binding; Systems Biophysics; titration; TRPP Cation Channels - chemistry; TRPP Cation Channels - metabolism

We present an improved and extended version of a recently proposed mathematical approach for modeling isotherms of ligand-to-macromolecule binding from isothermal titration calorimetry. Our approach uses ordinary differential equations, solved implicitly and numerically as initial value problems, to...