Isolation of Functional Human Endothelial Cells from Small Volumes of Umbilical Cord Blood

• Published in: Annals of biomedical engineering Vol. 41; no. 10; pp. 2181 - 2192
• Main Authors: Kang, Sa Do, Carlon, Tim A., Jantzen, Alexandra E., Lin, Fu-Hsiung, Ley, Melissa M., Allen, Jason D., Stabler, Thomas V., Haley, N. Rebecca, Truskey, George A., Achneck, Hardean E.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.10.2013; Springer Nature B.V
• Subjects: Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Biophysics; Cardiovascular diseases; Cells, Cultured; Centrifugation; Classical Mechanics; Endothelial Cells - cytology; Endothelial Cells - metabolism; Female; Fetal Blood - cytology; Fetal Blood - metabolism; Flow Cytometry - methods; Humans; Infant, Newborn; Male; Nitric oxide; Shear stress; Time Factors; Human serum; Platelet lysate; Late-outgrowth endothelial progenitor cell, EPC; Nitric oxide; EOC; Shear stress; ECFC; Cell differentiation; Cell isolation

Endothelial cells (ECs) isolated from endothelial progenitor cells in blood have great potential as a therapeutic tool to promote vasculogenesis and angiogenesis and treat cardiovascular diseases. However, current methods to isolate ECs are limited by a low yield with few colonies appearing during i...