Construction and characterization of an OmpH-deficient mutant of Pasteurella multocida strain X-73

• Published in: Avian Pathology Vol. 48; no. 1; pp. 4 - 11
• Main Authors: Varinrak, Thanya, Muenthaisong, Anucha, Apinda, Nisachon, Sawada, Takuo, Sthitmatee, Nattawooti
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.01.2019; Informa UK Limited; Taylor & Francis Ltd
• Subjects: agar; Animals; Antisense ompH; Antisense RNA; Bacteria; Bacterial Outer Membrane Proteins; Chickens; Colonies; Defective mutant; Deficient mutant; Dextrose; DNA, Antisense; Electroporation; Female; fowl cholera; genes; glucose; Homology; Inoculation; Mice; Microbiological strains; mutants; Mutation; Nucleic acids; outer membrane protein H; Pasteurella Infections; Pasteurella multocida; Pathogenicity; Pathogens; Poultry Diseases; Protection; Ribonucleic acid; RNA; serotypes; Starch; Strains (organisms); Survival; X-73; X-73; fowl cholera; outer membrane protein H; Antisense ompH; Pasteurella multocida

A capsule-defective mutant strain PBA129 of Pasteurella multocida was constructed by electroporation of phagemid containing the coding region of the antisense RNA of the ompH gene into the wild type strain X-73 (serovar A:1) of P. multocida. The pathogenicity and protective potency of the mutant aga...