Mesenchymal Stem/Stromal Cells in Three-Dimensional Cell Culture: Ion Homeostasis and Ouabain-Induced Apoptosis

• Published in: Biomedicines Vol. 11; no. 2; p. 301
• Main Authors: Shatrova, Alla N, Domnina, Alisa P, Pugovkina, Natalia A, Alekseenko, Larisa L, Marakhova, Irina I
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.01.2023; MDPI
• Subjects: Analysis; Apoptosis; Cardiac glycosides; Cell culture; Cell cycle; Cell growth; Endometrium; Experiments; Flow cytometry; Glycosides; Health aspects; Homeostasis; Membrane potential; Mesenchyme; Methods; Mitochondria; mitochondrial membrane potential; Na/K pump; Ouabain; potassium; Signal transduction; Spheroids; Stem cells; Stromal cells; three-dimensional spheroids; Water content; Wound healing; Russia; St Louis Missouri; United States > US; human endometrial mesenchymal stem/stromal cells; ouabain; three-dimensional spheroids; potassium; mitochondrial membrane potential; apoptosis; Na/K pump

This study describes the changes in ion homeostasis of human endometrial mesenchymal stem/stromal cells (eMSCs) during the formation of three-dimensional (3D) cell structures (spheroids) and investigates the conditions for apoptosis induction in 3D eMSCs. Detached from the monolayer culture, (2D) eM...