A deep sequencing tool for partitioning clearance rates following antimalarial treatment in polyclonal infections

• Published in: Evolution, medicine, and public health Vol. 2016; no. 1; pp. 21 - 36
• Main Authors: Mideo, Nicole, Bailey, Jeffrey A, Hathaway, Nicholas J, Ngasala, Billy, Saunders, David L, Lon, Chanthap, Kharabora, Oksana, Jamnik, Andrew, Balasubramanian, Sujata, Björkman, Anders, Mårtensson, Andreas, Meshnick, Steven R, Read, Andrew F, Juliano, Jonathan J
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 27.01.2016
• Subjects: Medicin och hälsovetenskap; Original; artemisinin; ecology; amplicon sequencing; within-host selection; drug resistance; malaria

Current tools struggle to detect drug-resistant malaria parasites when infections contain multiple parasite clones, which is the norm in high transmission settings in Africa. Our aim was to develop and apply an approach for detecting resistance that overcomes the challenges of polyclonal infections...