A Mouse-Adapted Enterovirus 71 Strain Causes Neurological Disease in Mice after Oral Infection

Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from...

Full description

Saved in:
Bibliographic Details
Published inJournal of Virology Vol. 78; no. 15; pp. 7916 - 7924
Main Authors WANG, Ya-Fang, CHOU, Chun-Ting, YU, Chun-Keung, LEI, Huan-Yao, LIU, Ching-Chuan, WANG, Shih-Min, YAN, Jing-Jou, SU, Ih-Jen, WANG, Jen-Reng, YEH, Trai-Ming, CHEN, Shun-Hua
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.08.2004
Subjects
5' Untranslated Regions - chemistry
Animals
Antibodies, Viral - immunology
Apoptosis
Biological and medical sciences
Central Nervous System Diseases - etiology
Central Nervous System Diseases - pathology
Enterovirus - pathogenicity
Enterovirus 71
Enterovirus Infections - pathology
Enterovirus Infections - virology
Fundamental and applied biological sciences. Psychology
Humans
Medical sciences
Mice
Mice, Inbred ICR
Microbiology
Miscellaneous
Pathogenesis and Immunity
Tumors
Virology
Virulence
Virus Replication
Picornaviridae
Motor system disorder
Size
Virulence
Central nervous system
Enterovirus
Neuroblastoma
Limb
Paralysis
Neurological disorder
Dose
Human
Nervous system diseases
Digestive system
Rodentia
Oral administration
Malignant tumor
In vitro
Strain
Virus
Infection
Vertebrata
Mammalia
Mouse
Cell death
Animal
Oral cavity disease
Autonomic neuropathy
Apoptosis
Online AccessGet full text

Cover

Abstract Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JVI .asm.org, visit: JVI       
AbstractList ABSTRACT A mouse-adapted enterovirus 71 (EV71) strain with increased virulence in mice, MP4, was generated after four serial passages of the parental EV71 strain 4643 in mice. Strain MP4 exhibited a larger plaque size, grew more rapidly, and was more cytotoxic in vitro than strain 4643. Although strains 4643 and MP4 both induced apoptosis of SK-N-SH human neuroblastoma cells, MP4 was more virulent than 4643 in 1-day-old mice (50% lethal doses, 10 2 and 10 4 PFU/mouse, respectively). Strain MP4 (5 × 10 6 PFU/mouse), but not 4643, could orally infect 7-day-old mice, resulting in rear-limb paralysis followed by death 5 to 9 days after inoculation with the virus. Histopathologically, neuronal loss and apoptosis were evident in the spinal cords as well as the brain stems of the infected mice. The limb muscles displayed massive necrosis. There was early and transient virus replication in the intestines, whereas the spinal cord, brain, and muscle became the sites of viral replication during the late phase of the infection. Virus transmission occurred among infected and noninfected cagemates, as demonstrated by the occurrence of seroconversion and the presence of viable viruses in the stool samples of the latter. Protection against EV71 challenge was demonstrated following administration of hyperimmune serum 1 day after inoculation with the virus. Nucleotide sequence analysis of the genome of EV71 strain MP4 revealed four nucleotide changes on the 5′ untranslated region, three on the VP2 region, and eight on the 2C region, resulting in one and four amino acid substitutions in the VP2 and 2C proteins, respectively.
A mouse-adapted enterovirus 71 (EV71) strain with increased virulence in mice, MP4, was generated after four serial passages of the parental EV71 strain 4643 in mice. Strain MP4 exhibited a larger plaque size, grew more rapidly, and was more cytotoxic in vitro than strain 4643. Although strains 4643 and MP4 both induced apoptosis of SK-N-SH human neuroblastoma cells, MP4 was more virulent than 4643 in 1-day-old mice (50% lethal doses, 10(2) and 10(4) PFU/mouse, respectively). Strain MP4 (5 x 10(6) PFU/mouse), but not 4643, could orally infect 7-day-old mice, resulting in rear-limb paralysis followed by death 5 to 9 days after inoculation with the virus. Histopathologically, neuronal loss and apoptosis were evident in the spinal cords as well as the brain stems of the infected mice. The limb muscles displayed massive necrosis. There was early and transient virus replication in the intestines, whereas the spinal cord, brain, and muscle became the sites of viral replication during the late phase of the infection. Virus transmission occurred among infected and noninfected cagemates, as demonstrated by the occurrence of seroconversion and the presence of viable viruses in the stool samples of the latter. Protection against EV71 challenge was demonstrated following administration of hyperimmune serum 1 day after inoculation with the virus. Nucleotide sequence analysis of the genome of EV71 strain MP4 revealed four nucleotide changes on the 5' untranslated region, three on the VP2 region, and eight on the 2C region, resulting in one and four amino acid substitutions in the VP2 and 2C proteins, respectively.
Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JVI .asm.org, visit: JVI       
A mouse-adapted enterovirus 71 (EV71) strain with increased virulence in mice, MP4, was generated after four serial passages of the parental EV71 strain 4643 in mice. Strain MP4 exhibited a larger plaque size, grew more rapidly, and was more cytotoxic in vitro than strain 4643. Although strains 4643 and MP4 both induced apoptosis of SK-N-SH human neuroblastoma cells, MP4 was more virulent than 4643 in 1-day-old mice (50% lethal doses, 10 2 and 10 4 PFU/mouse, respectively). Strain MP4 (5 × 10 6 PFU/mouse), but not 4643, could orally infect 7-day-old mice, resulting in rear-limb paralysis followed by death 5 to 9 days after inoculation with the virus. Histopathologically, neuronal loss and apoptosis were evident in the spinal cords as well as the brain stems of the infected mice. The limb muscles displayed massive necrosis. There was early and transient virus replication in the intestines, whereas the spinal cord, brain, and muscle became the sites of viral replication during the late phase of the infection. Virus transmission occurred among infected and noninfected cagemates, as demonstrated by the occurrence of seroconversion and the presence of viable viruses in the stool samples of the latter. Protection against EV71 challenge was demonstrated following administration of hyperimmune serum 1 day after inoculation with the virus. Nucleotide sequence analysis of the genome of EV71 strain MP4 revealed four nucleotide changes on the 5′ untranslated region, three on the VP2 region, and eight on the 2C region, resulting in one and four amino acid substitutions in the VP2 and 2C proteins, respectively.
A mouse-adapted enterovirus 71 (EV71) strain with increased virulence in mice, MP4, was generated after four serial passages of the parental EV71 strain 4643 in mice. Strain MP4 exhibited a larger plaque size, grew more rapidly, and was more cytotoxic in vitro than strain 4643. Although strains 4643 and MP4 both induced apoptosis of SK-N-SH human neuroblastoma cells, MP4 was more virulent than 4643 in 1-day-old mice (50% lethal doses, 10 super(2) and 10 super(4) PFU/mouse, respectively). Strain MP4 (5 x 10 super(6) PFU/mouse), but not 4643, could orally infect 7-day-old mice, resulting in rear-limb paralysis followed by death 5 to 9 days after inoculation with the virus. Histopathologically, neuronal loss and apoptosis were evident in the spinal cords as well as the brain stems of the infected mice. The limb muscles displayed massive necrosis. There was early and transient virus replication in the intestines, whereas the spinal cord, brain, and muscle became the sites of viral replication during the late phase of the infection. Virus transmission occurred among infected and noninfected cagemates, as demonstrated by the occurrence of seroconversion and the presence of viable viruses in the stool samples of the latter. Protection against EV71 challenge was demonstrated following administration of hyperimmune serum 1 day after inoculation with the virus. Nucleotide sequence analysis of the genome of EV71 strain MP4 revealed four nucleotide changes on the 5' untranslated region, three on the VP2 region, and eight on the 2C region, resulting in one and four amino acid substitutions in the VP2 and 2C proteins, respectively.
Author Shih-Min Wang
Shun-Hua Chen
Ya-Fang Wang
Ching-Chuan Liu
Ih-Jen Su
Chun-Keung Yu
Chun-Ting Chou
Trai-Ming Yeh
Jing-Jou Yan
Huan-Yao Lei
Jen-Reng Wang
AuthorAffiliation Institute of Basic Medical Sciences, 1 Departments of Microbiology and Immunology, 2 Pediatrics, 3 Pathology, 4 Clinical Medicine, 5 Medical Technology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China 6
AuthorAffiliation_xml – name: Institute of Basic Medical Sciences, 1 Departments of Microbiology and Immunology, 2 Pediatrics, 3 Pathology, 4 Clinical Medicine, 5 Medical Technology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China 6
Author_xml – sequence: 1
  givenname: Ya-Fang
  surname: WANG
  fullname: WANG, Ya-Fang
  organization: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 2
  givenname: Chun-Ting
  surname: CHOU
  fullname: CHOU, Chun-Ting
  organization: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 3
  givenname: Chun-Keung
  surname: YU
  fullname: YU, Chun-Keung
  organization: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 4
  givenname: Huan-Yao
  surname: LEI
  fullname: LEI, Huan-Yao
  organization: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 5
  givenname: Ching-Chuan
  surname: LIU
  fullname: LIU, Ching-Chuan
  organization: Department of Pediatrics, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 6
  givenname: Shih-Min
  surname: WANG
  fullname: WANG, Shih-Min
  organization: Department of Pediatrics, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 7
  givenname: Jing-Jou
  surname: YAN
  fullname: YAN, Jing-Jou
  organization: Department of Pathology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 8
  givenname: Ih-Jen
  surname: SU
  fullname: SU, Ih-Jen
  organization: Department of Clinical Medicine, Collegeof Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 9
  givenname: Jen-Reng
  surname: WANG
  fullname: WANG, Jen-Reng
  organization: Department of Medical Technology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 10
  givenname: Trai-Ming
  surname: YEH
  fullname: YEH, Trai-Ming
  organization: Department of Medical Technology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
– sequence: 11
  givenname: Shun-Hua
  surname: CHEN
  fullname: CHEN, Shun-Hua
  organization: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Province of China
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15969826$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/15254164$$D View this record in MEDLINE/PubMed
BookMark eNqFkU-PEyEYh4lZ43ZXv4IhJnqbEWaAgYOHpq5rza578E88SZiZl5bNFLowU7PfXpo2Wk9egOR9fvDyPhfozAcPCGFKSkor-fbT92XZyJLyslFUFI2qWFkRwp6gGSVKFpxTdoZmhFRVwWv54xxdpHRPCGVMsGfonPKKMyrYDP2c49swJSjmvdmO0OMrP0IMOxenhBuKv4zROI8XJjMJf4YphiGsXGcG_N4lMAlwLt-6DrCxOYnvYi4tvYVudME_R0-tGRK8OO6X6NuHq6-Lj8XN3fVyMb8pOi7UWFBlrG2kYRUDk9eWQWtFz5jsAHpat1b2lNXEStWqVrZU8nzkgsragmpZfYneHe7dTu0G-g587nvQ2-g2Jj7qYJz-t-LdWq_CTueB5IHl_JtjPoaHCdKoNy51MAzGQx6PFqKhVaPEf0HaKNXUss6gPIBdDClFsH-aoUTvJeosUTdSU673EvVeot5LzNGXp5_5Gzxay8DrI2BSNmGj8Z1LJ5wSSlb7Zl8duLVbrX-5CNqkjb7fuZN3698RLbRD
CitedBy_id crossref_primary_10_1007_s00705_012_1495_4
crossref_primary_10_1371_journal_ppat_1008142
crossref_primary_10_3389_fmicb_2017_02249
crossref_primary_10_3402_ehtj_v6i0_19780
crossref_primary_10_1016_j_virusres_2012_02_009
crossref_primary_10_1093_jac_dkw101
crossref_primary_10_1155_2014_878139
crossref_primary_10_1016_j_antiviral_2012_12_029
crossref_primary_10_1016_j_vaccine_2010_09_089
crossref_primary_10_1371_journal_pone_0057591
crossref_primary_10_1016_j_celrep_2023_112389
crossref_primary_10_1128_JVI_00692_14
crossref_primary_10_3390_v13081661
crossref_primary_10_1080_22221751_2020_1767512
crossref_primary_10_1371_journal_pone_0135154
crossref_primary_10_1016_j_jviromet_2011_02_003
crossref_primary_10_1007_s11262_010_0529_9
crossref_primary_10_1038_s41426_018_0201_3
crossref_primary_10_1038_srep36983
crossref_primary_10_3201_eid1811_111457
crossref_primary_10_1016_j_molcel_2021_10_003
crossref_primary_10_1038_emi_2017_49
crossref_primary_10_1016_j_jconrel_2016_10_019
crossref_primary_10_1186_1465_9921_12_147
crossref_primary_10_1016_j_virusres_2024_199386
crossref_primary_10_1128_JVI_00681_18
crossref_primary_10_1016_j_virol_2014_09_017
crossref_primary_10_3389_fmicb_2018_00817
crossref_primary_10_4103_idoj_idoj_423_22
crossref_primary_10_7774_cevr_2023_12_4_291
crossref_primary_10_1016_j_biopha_2019_109271
crossref_primary_10_1016_j_bjid_2015_06_011
crossref_primary_10_1073_pnas_1217563110
crossref_primary_10_4103_ijmm_IJMM_19_194
crossref_primary_10_1080_22221751_2021_1906755
crossref_primary_10_3390_v12060651
crossref_primary_10_1128_JVI_01627_15
crossref_primary_10_1002_bit_25625
crossref_primary_10_3390_v14020364
crossref_primary_10_1128_JVI_00183_19
crossref_primary_10_1016_j_vaccine_2011_06_044
crossref_primary_10_1007_s12250_013_3376_8
crossref_primary_10_1038_s41374_018_0075_y
crossref_primary_10_1099_vir_0_046383_0
crossref_primary_10_1016_j_antiviral_2017_06_008
crossref_primary_10_1007_s00705_011_1198_2
crossref_primary_10_1038_s41598_019_47455_3
crossref_primary_10_1128_JVI_03498_14
crossref_primary_10_1126_sciadv_aau4255
crossref_primary_10_1186_1471_2180_12_162
crossref_primary_10_1016_j_virol_2010_12_058
crossref_primary_10_1038_s41374_020_0456_x
crossref_primary_10_1016_j_virusres_2015_11_007
crossref_primary_10_1099_vir_0_022418_0
crossref_primary_10_1007_s00430_013_0289_y
crossref_primary_10_3390_ijms24010169
crossref_primary_10_7774_cevr_2023_0343
crossref_primary_10_3389_fmicb_2022_821976
crossref_primary_10_1128_JVI_01062_17
crossref_primary_10_1016_j_micinf_2023_105107
crossref_primary_10_3390_ijms20205201
crossref_primary_10_3389_fimmu_2021_753683
crossref_primary_10_1371_journal_pone_0092719
crossref_primary_10_1016_j_virol_2009_10_017
crossref_primary_10_1186_s12929_015_0181_8
crossref_primary_10_1002_smll_202002435
crossref_primary_10_1080_22221751_2020_1729665
crossref_primary_10_1371_journal_pbio_3001693
crossref_primary_10_1111_bpa_12279
crossref_primary_10_1155_2018_8475463
crossref_primary_10_1002_rmv_538
crossref_primary_10_1016_j_jprot_2015_05_016
crossref_primary_10_1371_journal_pone_0111174
crossref_primary_10_3390_v9110351
crossref_primary_10_1099_vir_0_80784_0
crossref_primary_10_1128_JVI_03826_13
crossref_primary_10_1093_jac_dkq301
crossref_primary_10_1021_pr501199h
crossref_primary_10_3390_v8010011
crossref_primary_10_1128_JVI_00372_07
crossref_primary_10_1186_s12929_020_0615_9
crossref_primary_10_3390_pathogens9020121
crossref_primary_10_1371_journal_ppat_1011967
crossref_primary_10_1128_JVI_00999_09
crossref_primary_10_1186_s12929_014_0080_4
crossref_primary_10_1038_s41419_022_04771_3
crossref_primary_10_1128_mBio_02712_21
crossref_primary_10_1002_iub_1434
crossref_primary_10_1016_j_coviro_2012_02_009
crossref_primary_10_1111_j_1469_0691_2007_01729_x
crossref_primary_10_1038_emi_2016_56
crossref_primary_10_1016_j_carbpol_2015_12_005
crossref_primary_10_1016_j_vaccine_2019_07_046
crossref_primary_10_4015_S101623720600049X
crossref_primary_10_1002_rmv_2073
crossref_primary_10_1111_prd_12273
crossref_primary_10_1371_journal_pone_0147463
crossref_primary_10_1038_s41598_017_12388_2
crossref_primary_10_1038_s41598_017_01110_x
crossref_primary_10_1128_JVI_02090_13
crossref_primary_10_1371_journal_ppat_1006375
crossref_primary_10_1016_j_jbc_2022_101850
crossref_primary_10_1128_AAC_01922_19
crossref_primary_10_1186_s12929_023_00908_4
crossref_primary_10_1016_j_virusres_2022_198692
crossref_primary_10_1128_JVI_01779_10
crossref_primary_10_2217_fmb_12_22
crossref_primary_10_1186_s12929_019_0574_1
crossref_primary_10_3892_mmr_2017_7142
crossref_primary_10_3390_v13010058
crossref_primary_10_1099_jgv_0_000640
crossref_primary_10_1371_journal_ppat_1008428
crossref_primary_10_1080_15384101_2019_1704512
crossref_primary_10_1007_s11684_012_0237_z
crossref_primary_10_1128_JVI_00236_07
crossref_primary_10_1371_journal_pone_0109391
crossref_primary_10_1186_s12985_017_0872_8
crossref_primary_10_1038_aps_2016_110
crossref_primary_10_1371_journal_pone_0051996
crossref_primary_10_1007_s10875_021_01170_9
crossref_primary_10_1016_j_vaccine_2021_02_024
crossref_primary_10_1186_1743_422X_2_74
crossref_primary_10_1099_vir_0_043893_0
crossref_primary_10_1371_journal_pone_0027082
crossref_primary_10_1038_ncomms4344
crossref_primary_10_1007_s11262_014_1035_2
crossref_primary_10_1371_journal_ppat_1004613
crossref_primary_10_1016_j_virusres_2012_04_009
crossref_primary_10_1097_NEN_0b013e31817713e7
crossref_primary_10_1371_journal_pone_0077133
crossref_primary_10_3390_v12080867
crossref_primary_10_1016_j_jviromet_2015_08_007
crossref_primary_10_1002_jmv_25525
crossref_primary_10_1038_srep34299
crossref_primary_10_3390_v10120689
crossref_primary_10_3390_v7112916
crossref_primary_10_1016_j_medmic_2020_100006
crossref_primary_10_1128_JVI_02057_15
crossref_primary_10_1128_JVI_02057_18
crossref_primary_10_1371_journal_pone_0065948
crossref_primary_10_1128_mSphere_01048_20
crossref_primary_10_1016_j_virol_2011_10_015
crossref_primary_10_1371_journal_pone_0136420
crossref_primary_10_1038_srep24149
crossref_primary_10_1016_j_micpath_2020_104603
crossref_primary_10_1007_s00705_022_05606_4
crossref_primary_10_1038_s41598_018_30631_2
crossref_primary_10_3390_v13020308
crossref_primary_10_1016_j_virol_2014_07_021
crossref_primary_10_1371_journal_pone_0069858
crossref_primary_10_1097_NEN_0b013e31816564f0
crossref_primary_10_1371_journal_pntd_0002538
crossref_primary_10_3390_v10120674
crossref_primary_10_1186_1743_422X_10_28
crossref_primary_10_1016_j_biopha_2017_09_117
crossref_primary_10_1016_j_tim_2005_11_003
crossref_primary_10_1007_s12250_010_3102_8
crossref_primary_10_1016_j_virusres_2018_07_008
crossref_primary_10_3389_fimmu_2020_614743
crossref_primary_10_2217_fvl_10_22
crossref_primary_10_1128_mSphere_00377_20
crossref_primary_10_1002_jmv_21502
crossref_primary_10_1038_srep28876
crossref_primary_10_7243_2050_1412_3_1
crossref_primary_10_1186_1743_422X_10_215
crossref_primary_10_1371_journal_pntd_0003692
crossref_primary_10_1128_mBio_02540_20
crossref_primary_10_1016_j_micinf_2011_04_004
crossref_primary_10_11569_wcjd_v27_i24_1465
crossref_primary_10_1371_journal_pone_0010735
crossref_primary_10_1016_j_vaccine_2010_08_080
crossref_primary_10_4167_jbv_2022_52_1_020
crossref_primary_10_1002_jmv_23377
crossref_primary_10_1038_labinvest_2011_82
crossref_primary_10_1016_S1875_9572_08_60024_8
crossref_primary_10_1186_s12985_017_0911_5
crossref_primary_10_1038_s41564_023_01339_5
crossref_primary_10_1016_j_antiviral_2014_06_004
crossref_primary_10_1016_j_biologicals_2016_01_003
crossref_primary_10_1371_journal_pone_0095272
crossref_primary_10_1016_j_virusres_2014_05_006
crossref_primary_10_1186_1743_422X_7_47
crossref_primary_10_1186_s12929_019_0585_y
crossref_primary_10_1016_j_diagmicrobio_2007_07_010
crossref_primary_10_1099_vir_0_83676_0
crossref_primary_10_1038_emi_2013_44
crossref_primary_10_1186_s12929_023_00987_3
crossref_primary_10_1016_j_vaccine_2011_01_094
crossref_primary_10_1086_527326
crossref_primary_10_1099_vir_0_81195_0
crossref_primary_10_1016_j_antiviral_2016_11_008
crossref_primary_10_1016_j_vaccine_2009_03_060
crossref_primary_10_1007_s00705_011_1222_6
crossref_primary_10_1016_j_chom_2010_12_001
crossref_primary_10_1186_1423_0127_21_31
crossref_primary_10_1111_cmi_12401
crossref_primary_10_3892_etm_2017_4499
crossref_primary_10_1186_s12866_015_0474_9
crossref_primary_10_1016_S1473_3099_10_70194_8
crossref_primary_10_1038_srep31151
crossref_primary_10_1038_emi_2014_35
crossref_primary_10_1371_journal_pone_0119173
crossref_primary_10_1371_journal_pone_0193155
crossref_primary_10_1371_journal_pone_0063431
crossref_primary_10_1128_jvi_00561_22
crossref_primary_10_4161_15384101_2014_980631
crossref_primary_10_3389_fimmu_2020_561758
crossref_primary_10_1128_JVI_01798_07
crossref_primary_10_1128_JVI_00897_21
crossref_primary_10_1371_journal_pone_0059501
crossref_primary_10_1002_jmv_22198
crossref_primary_10_2217_fvl_12_89
crossref_primary_10_1128_JVI_00434_09
crossref_primary_10_1155_2012_580696
crossref_primary_10_1016_j_virol_2023_02_002
crossref_primary_10_1111_cmi_13043
Cites_doi 10.1111/j.1574-6976.2002.tb00601.x
10.1086/338819
10.3201/eid1301.020112
10.1128/JVI.77.1.115-122.2003
10.1016/S0042-6822(03)00544-0
10.1093/infdis/169.4.905
10.1086/345905
10.1099/0022-1317-83-6-1367
10.1128/JVI.76.21.10617-10626.2002
10.1128/jvi.74.10.4787-4794.2000
10.1002/jmv.2038
10.1099/vir.0.19423-0
10.1128/jvi.71.5.3826-3833.1997
10.1002/jmv.1890470209
10.1128/jvi.71.5.3986-3991.1997
10.1086/319255
10.1086/376998
10.1056/NEJM199909233411302
10.1099/0022-1317-73-12-3065
10.1002/jmv.2209
10.1128/jvi.69.8.4607-4618.1995
10.1007/BF01317504
10.1111/j.1365-2990.1982.tb00269.x
10.1111/j.1651-2227.2002.tb03292.x
10.1016/S1386-6532(00)00068-8
10.1006/viro.2001.1310
10.1086/520149
10.1056/NEJM199909233411301
10.1007/BF02253368
10.1212/01.WNL.0000066810.62490.FF
ContentType Journal Article
Copyright 2004 INIST-CNRS
Copyright © 2004, American Society for Microbiology 2004
Copyright_xml – notice: 2004 INIST-CNRS
– notice: Copyright © 2004, American Society for Microbiology 2004
DBID IQODW
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7U9
H94
7X8
5PM
DOI 10.1128/JVI.78.15.7916-7924.2004
DatabaseName Pascal-Francis
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Virology and AIDS Abstracts
AIDS and Cancer Research Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
AIDS and Cancer Research Abstracts
Virology and AIDS Abstracts
MEDLINE - Academic
DatabaseTitleList CrossRef
MEDLINE


AIDS and Cancer Research Abstracts
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1098-5514
EndPage 7924
ExternalDocumentID 10_1128_JVI_78_15_7916_7924_2004
15254164
15969826
jvi_78_15_7916
Genre Journal Article
GroupedDBID ---
-~X
.55
.GJ
08R
0R~
18M
29L
2WC
39C
3O-
4.4
41~
53G
5GY
5RE
5VS
6TJ
85S
AAUGY
AAYJJ
ABPPZ
ABPTK
ACGFO
ACNCT
ADBBV
AENEX
AFDAS
AFFNX
AI.
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BTFSW
C1A
CS3
D0S
DIK
E3Z
EBS
EJD
F20
F5P
FRP
GX1
H13
HYE
HZ~
IH2
IQODW
KQ8
MVM
N9A
O9-
OHT
OK1
P2P
RHF
RHI
RNS
RPM
RSF
TR2
UCJ
UPT
VH1
W2D
W8F
WH7
WOQ
X7M
XFK
Y6R
YQT
ZA5
ZGI
ZXP
~02
~KM
AGVNZ
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7U9
H94
7X8
5PM
ID FETCH-LOGICAL-c569t-19aff78a424eaa42b4ebf6d448ceed13bf8d1430f89b9b8b185f8956183fe9b43
IEDL.DBID RPM
ISSN 0022-538X
IngestDate Tue Sep 17 21:00:49 EDT 2024
Fri Oct 25 10:48:38 EDT 2024
Fri Oct 25 04:11:05 EDT 2024
Thu Sep 12 19:51:00 EDT 2024
Sat Sep 28 07:40:34 EDT 2024
Sun Oct 22 16:02:31 EDT 2023
Wed May 18 15:31:24 EDT 2016
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 15
Keywords Picornaviridae
Motor system disorder
Size
Virulence
Central nervous system
Enterovirus
Neuroblastoma
Limb
Paralysis
Neurological disorder
Dose
Human
Nervous system diseases
Digestive system
Rodentia
Oral administration
Malignant tumor
In vitro
Strain
Virus
Infection
Vertebrata
Mammalia
Mouse
Cell death
Animal
Oral cavity disease
Autonomic neuropathy
Apoptosis
Language English
License CC BY 4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c569t-19aff78a424eaa42b4ebf6d448ceed13bf8d1430f89b9b8b185f8956183fe9b43
Notes ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Corresponding author. Mailing address: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China. Phone: 886-6-2353535, ext. 5673. Fax: 886-6-2082705. E-mail: dckyu@mail.ncku.edu.tw.
OpenAccessLink https://europepmc.org/articles/pmc446098?pdf=render
PMID 15254164
PQID 17997383
PQPubID 23462
PageCount 9
ParticipantIDs pubmed_primary_15254164
highwire_asm_jvi_78_15_7916
crossref_primary_10_1128_JVI_78_15_7916_7924_2004
pascalfrancis_primary_15969826
proquest_miscellaneous_66712796
proquest_miscellaneous_17997383
pubmedcentral_primary_oai_pubmedcentral_nih_gov_446098
PublicationCentury 2000
PublicationDate 2004-08-01
PublicationDateYYYYMMDD 2004-08-01
PublicationDate_xml – month: 08
  year: 2004
  text: 2004-08-01
  day: 01
PublicationDecade 2000
PublicationPlace Washington, DC
PublicationPlace_xml – name: Washington, DC
– name: United States
PublicationTitle Journal of Virology
PublicationTitleAlternate J Virol
PublicationYear 2004
Publisher American Society for Microbiology
Publisher_xml – name: American Society for Microbiology
References 14718621 - J Gen Virol. 2004 Jan;85(Pt 1):69-77
9094658 - J Virol. 1997 May;71(5):3826-33
1335021 - J Gen Virol. 1992 Dec;73 ( Pt 12):3065-77
10775617 - J Virol. 2000 May;74(10):4787-94
11938497 - Clin Infect Dis. 2002 May 1;34 Suppl 2:S52-7
12368304 - J Virol. 2002 Nov;76(21):10617-26
8133108 - J Infect Dis. 1994 Apr;169(4):905-8
11992581 - J Med Virol. 2002 Jun;67(2):207-16
7048123 - Neuropathol Appl Neurobiol. 1982 Mar-Apr;8(2):149-56
10498488 - N Engl J Med. 1999 Sep 23;341(13):936-42
11060501 - J Biomed Sci. 2000 Nov-Dec;7(6):523-8
12007645 - FEMS Microbiol Rev. 2002 Mar;26(1):91-107
11886259 - Virology. 2002 Feb 15;293(2):386-95
8830120 - J Med Virol. 1995 Oct;47(2):161-7
12898444 - J Infect Dis. 2003 Aug 15;188(4):564-70
10814935 - J Clin Virol. 2000 Jun;17(1):23-30
12771257 - Neurology. 2003 May 27;60(10):1651-6
14592777 - Virology. 2003 Oct 10;315(1):259-66
9094675 - J Virol. 1997 May;71(5):3986-91
228639 - Arch Virol. 1979;60(3-4):329-40
12477816 - J Virol. 2003 Jan;77(1):115-22
12162592 - Acta Paediatr. 2002;91(6):632-5
12539066 - Clin Infect Dis. 2003 Feb 1;36(3):269-74
10433583 - Clin Infect Dis. 1999 Jul;29(1):184-90
12029152 - J Gen Virol. 2002 Jun;83(Pt 6):1367-76
12533285 - Emerg Infect Dis. 2003 Jan;9(1):78-85
7609025 - J Virol. 1995 Aug;69(8):4607-18
10498487 - N Engl J Med. 1999 Sep 23;341(13):929-35
11536241 - J Med Virol. 2001 Oct;65(2):331-9
11237800 - J Infect Dis. 2001 Mar 15;183(6):850-6
e_1_3_1_21_2
e_1_3_1_22_2
e_1_3_1_23_2
e_1_3_1_8_2
e_1_3_1_7_2
e_1_3_1_9_2
e_1_3_1_20_2
(e_1_3_1_25_2) 1938; 27
e_1_3_1_4_2
e_1_3_1_29_2
e_1_3_1_3_2
e_1_3_1_6_2
e_1_3_1_5_2
(e_1_3_1_24_2) 2001; 1
e_1_3_1_26_2
e_1_3_1_2_2
e_1_3_1_27_2
e_1_3_1_28_2
e_1_3_1_32_2
e_1_3_1_33_2
e_1_3_1_34_2
e_1_3_1_13_2
e_1_3_1_12_2
e_1_3_1_11_2
e_1_3_1_30_2
e_1_3_1_10_2
e_1_3_1_31_2
e_1_3_1_17_2
e_1_3_1_16_2
e_1_3_1_15_2
e_1_3_1_14_2
e_1_3_1_19_2
e_1_3_1_18_2
References_xml – ident: e_1_3_1_19_2
  doi: 10.1111/j.1574-6976.2002.tb00601.x
– ident: e_1_3_1_14_2
  doi: 10.1086/338819
– ident: e_1_3_1_3_2
  doi: 10.3201/eid1301.020112
– ident: e_1_3_1_23_2
  doi: 10.1128/JVI.77.1.115-122.2003
– ident: e_1_3_1_27_2
  doi: 10.1016/S0042-6822(03)00544-0
– ident: e_1_3_1_2_2
  doi: 10.1093/infdis/169.4.905
– ident: e_1_3_1_16_2
  doi: 10.1086/345905
– ident: e_1_3_1_12_2
  doi: 10.1099/0022-1317-83-6-1367
– volume: 1
  start-page: 685
  year: 2001
  ident: e_1_3_1_24_2
  publication-title: Fields virology, 4th ed.
– volume: 27
  start-page: 493
  year: 1938
  ident: e_1_3_1_25_2
  publication-title: Am. J. Hyg.
– ident: e_1_3_1_18_2
  doi: 10.1128/JVI.76.21.10617-10626.2002
– ident: e_1_3_1_6_2
  doi: 10.1128/jvi.74.10.4787-4794.2000
– ident: e_1_3_1_31_2
  doi: 10.1002/jmv.2038
– ident: e_1_3_1_4_2
  doi: 10.1099/vir.0.19423-0
– ident: e_1_3_1_7_2
  doi: 10.1128/jvi.71.5.3826-3833.1997
– ident: e_1_3_1_34_2
  doi: 10.1002/jmv.1890470209
– ident: e_1_3_1_26_2
  doi: 10.1128/jvi.71.5.3986-3991.1997
– ident: e_1_3_1_32_2
  doi: 10.1086/319255
– ident: e_1_3_1_29_2
  doi: 10.1086/376998
– ident: e_1_3_1_11_2
  doi: 10.1056/NEJM199909233411302
– ident: e_1_3_1_20_2
  doi: 10.1099/0022-1317-73-12-3065
– ident: e_1_3_1_21_2
  doi: 10.1002/jmv.2209
– ident: e_1_3_1_28_2
  doi: 10.1128/jvi.69.8.4607-4618.1995
– ident: e_1_3_1_5_2
  doi: 10.1007/BF01317504
– ident: e_1_3_1_8_2
  doi: 10.1111/j.1365-2990.1982.tb00269.x
– ident: e_1_3_1_15_2
  doi: 10.1111/j.1651-2227.2002.tb03292.x
– ident: e_1_3_1_17_2
  doi: 10.1016/S1386-6532(00)00068-8
– ident: e_1_3_1_13_2
  doi: 10.1006/viro.2001.1310
– ident: e_1_3_1_30_2
  doi: 10.1086/520149
– ident: e_1_3_1_9_2
  doi: 10.1056/NEJM199909233411301
– ident: e_1_3_1_33_2
  doi: 10.1007/BF02253368
– ident: e_1_3_1_22_2
  doi: 10.1212/01.WNL.0000066810.62490.FF
– ident: e_1_3_1_10_2
SSID ssj0014464
Score 2.3160636
Snippet Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
A mouse-adapted enterovirus 71 (EV71) strain with increased virulence in mice, MP4, was generated after four serial passages of the parental EV71 strain 4643...
ABSTRACT A mouse-adapted enterovirus 71 (EV71) strain with increased virulence in mice, MP4, was generated after four serial passages of the parental EV71...
SourceID pubmedcentral
proquest
crossref
pubmed
pascalfrancis
highwire
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 7916
SubjectTerms 5' Untranslated Regions - chemistry
Animals
Antibodies, Viral - immunology
Apoptosis
Biological and medical sciences
Central Nervous System Diseases - etiology
Central Nervous System Diseases - pathology
Enterovirus - pathogenicity
Enterovirus 71
Enterovirus Infections - pathology
Enterovirus Infections - virology
Fundamental and applied biological sciences. Psychology
Humans
Medical sciences
Mice
Mice, Inbred ICR
Microbiology
Miscellaneous
Pathogenesis and Immunity
Tumors
Virology
Virulence
Virus Replication
Title A Mouse-Adapted Enterovirus 71 Strain Causes Neurological Disease in Mice after Oral Infection
URI http://jvi.asm.org/content/78/15/7916.abstract
https://www.ncbi.nlm.nih.gov/pubmed/15254164
https://search.proquest.com/docview/17997383
https://search.proquest.com/docview/66712796
https://pubmed.ncbi.nlm.nih.gov/PMC446098
Volume 78
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1db9MwFL2ik5B4QTC-wljnB16dLokT249V2adUQIKhPmHZiS2KaFYt3ST-Pdd2Ai2Cl71UkexYjc91fI58ci_AWyN47cGljckbyhopqCiYoY47x2WNESb918jz99X5FbtclIv-o7Cut1W2tVmm7Y9V2i6_BW_lelVPBp_Y5ON8hhLmWIrJCEYYn4NC708OsI0NGcJxMS8G904uJpdfLlIu0qxMOZIiylF6BIEYS_OUSE3Y7u40ZAz2hknd4Zy5WOziX2z0b1Pl1i51-gQe9_SSTONjPIUHtt2Hh7Hg5M9n8HVK5ij1LZ02eo1ckwRPwPXd8ua2Izwjn0LFCDLT2KcjIXFH_24k7-JJDsHmOb5cSCguTj7gfyAXvaGrfQ5XpyefZ-e0r7BA67KSG5pJjZAIzXJmNf4aZo2rGpRsfu_MCuNEg4Tq2AlppBEGN3e8RMolCmelYcUL2GuvW_sKiGZ1wVzFeYGMQQurTVMbzspGFz6_T55ANsysWsdEGioIkFwoBEZxobJSeWCUB8aXx2QJHAwQKN2t1Pe75Va_BMY7qPwZtpSVRNmUwNEAk8JF409CdGtxlpVPg8dRm_-_R1XxLOcSx3gZYd0aPYZKAtUO4L87-ITduy0YxyFxd4zb1_e98QAeRd-Qtx--gb3Nza09REq0MWMYnS2ycVgJvwDrlgpW
link.rule.ids 230,315,730,783,787,888,27937,27938,53805,53807
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb9MwFH6CIQSX8XMsDDYfuCZtEie2j1XZ1I5lILGinrDsJNY6aFot7ST463mOk9FOcIBLFOk5luLv2f6e_Pk9gHeas9yC6xc6KnxaCO7zmGrfMGOYyNHDhL2NnJ2nowk9nSbT9lJY3coqq1zPgur7PKhml422cjnPe51OrPcpG2II0xe8dx8e4HTtJ12M3p4doJV2OcLRPu30OxHvnX4ZB4wHYRIwpEU-w-CjCRFdcZ4EyQnd3p-6nMFWMqlqHDXjyl38iY_elVVu7FMnT2DS_aGTp3wL1isd5D_vJH_81yF4CrstcSUDZ30G98rqOTx0pSx_vICvA5It1nXpDwq1RBZLGrXB4mZ2va4JC8nnphYFGSpsU5MmJUi76pL37oyIoDnDZYs0ZcvJR_w3Mm6lYtVLmJwcXwxHflu7wc-TVKz8UCgEmysa0VLhU9NSm7TAYNDuymGsDS-QqvUNF1porpE24CuSOR6bUmga78FOtajKfSCK5jE1KWMxchHFS6WLXDOaFCq2mYMiD8IOMbl0KTpkE9pEXCLgknEZJtICLi3gtvAm9eCgg1aqei6vbmYb7Tw43EL7d7eJSAUGZB4cdfBLnI72jEVVJY6ytAn2GEb9f2-RpiyMmMA-Xjl32ejduaAH6ZYj3TawqcC3LegeTUpw5w6v__fDI3g0usjO5Nn4_MMBPHbqJCtyfAM7q-t1-RaJ10ofNvPsFwGWKyo
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb9MwFH4aQyAu_B6EweYD1yRL4sT2seqo1kHHJBgqFyw7iUWBptXSToK_nmc7gXaCyy5RJL9Yit-z_T358_cAXmvOSuvcsNJpFdJK8JBnVIeGGcNEiREm7G3kyVlxckFPp_l0B3h_F8aR9ks9i5of86iZfXXcyuW8jHueWHw-GWIKcyR4vKxMfAtu51Yzvc_Tu_MDtKC9Tji2T3sOT8rj00_jiPEoySOG0ChkmIC4NNEX6MkRoNDtParXDba0SdXiyBlf8uJfmPQ6tXJjrxo9gM_9X3qKyvdovdJR-euaAORNhuEh3O8ALBl4i0ewUzeP4Y4vafnzCXwZkMli3dbhoFJLRLPEsQ4WV7PLdUtYQj64mhRkqNCmJU4apFt9ybE_KyLYPMHli7jy5eQ9_h8Zd5Sx5ilcjN58HJ6EXQ2HsMwLsQoTodDpXNGU1gqfmtbaFBUmhXZ3TjJteIWQ7chwoYXmGuEDviKo45mphabZHuw2i6Z-DkTRMqOmYCxDTKJ4rXRVakbzSmVWQSgNIOm9JpdeqkO6FCflEp0uGZdJLq3TpXW6LcBJA9jv3StVO5ffrmYbdgEcbHn8b7e5KAQmZgEc9iEgcVrasxbV1DjK0grtMcz-_29RFCxJmcA-nvmQ2ejdh2EAxVYw_TGwkuDbLRgiThrch8SLm354CHfPj0fy3fjs7T7c8yQly3V8Cbury3X9CvHXSh-4qfYbYHYtqg
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Mouse-Adapted+Enterovirus+71+Strain+Causes+Neurological+Disease+in+Mice+after+Oral+Infection&rft.jtitle=Journal+of+virology&rft.au=Wang%2C+Ya-Fang&rft.au=Chou%2C+Chun-Ting&rft.au=Lei%2C+Huan-Yao&rft.au=Liu%2C+Ching-Chuan&rft.date=2004-08-01&rft.pub=American+Society+for+Microbiology&rft.issn=0022-538X&rft.eissn=1098-5514&rft.volume=78&rft.issue=15&rft.spage=7916&rft.epage=7924&rft_id=info:doi/10.1128%2FJVI.78.15.7916-7924.2004&rft_id=info%3Apmid%2F15254164&rft.externalDBID=PMC446098
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-538X&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-538X&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-538X&client=summon