Homology-mediated end joining-based targeted integration using CRISPR/Cas9

• Published in: Cell research Vol. 27; no. 6; pp. 801 - 814
• Main Authors: Yao, Xuan, Wang, Xing, Hu, Xinde, Liu, Zhen, Liu, Junlai, Zhou, Haibo, Shen, Xiaowen, Wei, Yu, Huang, Zijian, Ying, Wenqin, Wang, Yan, Nie, Yan-Hong, Zhang, Chen-Chen, Li, Sanlan, Cheng, Leping, Wang, Qifang, Wu, Yan, Huang, Pengyu, Sun, Qiang, Shi, Linyu, Yang, Hui
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.06.2017; Nature Publishing Group
• Subjects: 631/1647/1511; 631/1647/1513/1967/3196; 631/337/1427; 631/61/201; Animal models; Animals; Biomedical and Life Sciences; Cell Biology; CRISPR-Cas Systems - genetics; CRISPR-Cas Systems - physiology; DNA End-Joining Repair - genetics; DNA End-Joining Repair - physiology; Embryos; Gene Knock-In Techniques; Genetic Engineering - methods; HEK293; HEK293 Cells; Hepatocytes - metabolism; Humans; Life Sciences; Mice; Original; original-article; RNA, Guide, CRISPR-Cas Systems - genetics; Stem cells; 一体化; 人力资源; 介导; 外源基因; 定点整合; 小鼠胚胎干细胞; 非同源末端连接; monkey embryos; homology-mediated end joining; CRISPR/Cas9; neurons; knock-in

Targeted integration of transgenes can be achieved by strategies based on homologous recombination （HR）, mi- crohomology-mediated end joining （MMEJ） or non-homologous end joining （NHEJ）. The more generally used HR is inefficient for achieving gene integration in animal embryos and tissues, because i...