WDR33 alternative polyadenylation is dependent on stochastic poly(a) site usage and splicing efficiencies

• Published in: RNA biology Vol. 21; no. 1; pp. 970 - 980
• Main Authors: Liu, Lizhi, Seimiya, Takahiro, Manley, James L.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 31.12.2024; Taylor & Francis Group
• Subjects: alternative polyadenylation; Alternative Splicing; CFIm25; CRISPR/Cas13; Exons; Humans; innate immune responses; innate immunity; Introns; minigene; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Poly A - genetics; Poly A - metabolism; Polyadenylation; polyadenylation site; protein isoforms; Protein Isoforms - genetics; Protein Isoforms - metabolism; Research Paper; RNA; RNA Splicing; RNA, Messenger - genetics; RNA, Messenger - metabolism; WDR33; alternative splicing; minigene; CFIm25; CRISPR/Cas13; polyadenylation site; innate immune responses; WDR33; alternative polyadenylation

Transcripts from the human WDR33 gene, which encodes a central component of the mRNA polyadenylation (PA) machinery, are subject to alternative polyadenylation (APA) within promoter-proximal introns/exons. This APA, which itself involves usage of multiple PA sites, results in the production of two n...