Dose-dependent modulation of the T cell proteome by ascorbic acid

• Published in: British journal of nutrition Vol. 97; no. 1; pp. 19 - 26
• Main Authors: Grant, Melissa M., Mistry, Nalini, Lunec, Joseph, Griffiths, Helen R.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 2007
• Subjects: Acids; Antioxidants; Apoptosis; Ascorbic acid; Ascorbic Acid - pharmacology; Biological and medical sciences; Blotting, Western; Blotting, Western - methods; Carbohydrate metabolism; Carbohydrates; Cell cycle; Cell Line, Transformed; chemistry; culture media; cultured cells; Dilution; DNA Fingerprinting; dosage; dose response; Dose-Response Relationship, Drug; drug effects; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; Feeding. Feeding behavior; Fluorides; Fundamental and applied biological sciences. Psychology; gene expression; genetics; genome; Genomes; genomics; Humans; Immune response; In vivo methods and tests; Intracellular signalling; Kinases; Lymphocytes; Lymphocytes T; Membranes; metabolism; methods; Monoclonal antibodies; Oxidative stress; pharmacology; Phosphatidylinositol; Phosphatidylinositol transfer protein; Phosphoinositol transfer protein; Phospholipid Transfer Proteins; Phospholipid Transfer Proteins - genetics; Phospholipid Transfer Proteins - metabolism; Protein folding; protein synthesis; Proteins; proteome; Proteome - drug effects; Proteome - genetics; Proteome - metabolism; Proteomes; Proteomics; signal transduction; Signal Transduction - drug effects; Signal Transduction - genetics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; T cells; T-lymphocytes; T-Lymphocytes - chemistry; T-Lymphocytes - drug effects; T-Lymphocytes - metabolism; Target recognition; Time Factors; transcription (genetics); Vertebrates: anatomy and physiology, studies on body, several organs or systems; Western blotting; United Kingdom > UK; United States > US; Phosphoinositol transfer protein; Ascorbic acid; T cells; Proteomics; Vertebrata; Ascorbic acid: T cells: Proteomics: Phosphoinositol transfer protein; Mammalia; Enzyme; Transferases; Modulation; T-Lymphocyte; Transfer; Aminomethyltransferase; Dose
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1017/S0007114507197592

To investigate the hypothesis that the micronutrient ascorbic acid can modulate the functional genome, T cells (CCRF-HSB2) were treated with ascorbic acid (up to 150 μm) for up to 24 h. Protein expression changes were assessed by two-dimensional electrophoresis. Forty-one protein spots which showed greater than two-fold expression changes were subject to identification by matrix-assisted laser desorption ionisation time of flight MS. The confirmed protein identifications were clustered into five groups; proteins were associated with signalling, carbohydrate metabolism, apoptosis, transcription and immune function. The increased expression of phosphatidylinositol transfer protein (promotes intracellular signalling) within 5 min of ascorbic acid treatment was confirmed by Western blotting. Together, these observations suggest that ascorbic acid modulates the T cell proteome in a time- and dose-dependent manner and identify molecular targets for study following antioxidant supplementation in vivo.