Shark nanobodies with potent SARS-CoV-2 neutralizing activity and broad sarbecovirus reactivity

• Published in: Nature communications Vol. 14; no. 1; pp. 580 - 18
• Main Authors: Chen, Wei-Hung, Hajduczki, Agnes, Martinez, Elizabeth J., Bai, Hongjun, Matz, Hanover, Hill, Thomas M., Lewitus, Eric, Chang, William C., Dawit, Layla, Peterson, Caroline E., Rees, Phyllis A., Ajayi, Adelola B., Golub, Emily S., Swafford, Isabella, Dussupt, Vincent, David, Sapna, Mayer, Sandra V., Soman, Sandrine, Kuklis, Caitlin, Corbitt, Courtney, King, Jocelyn, Choe, Misook, Sankhala, Rajeshwer S., Thomas, Paul V., Zemil, Michelle, Wieczorek, Lindsay, Hart, Tricia, Duso, Debora, Kummer, Larry, Yan, Lianying, Sterling, Spencer L., Laing, Eric D., Broder, Christopher C., Williams, Jazmean K., Davidson, Edgar, Doranz, Benjamin J., Krebs, Shelly J., Polonis, Victoria R., Paquin-Proulx, Dominic, Rolland, Morgane, Reiley, William W., Gromowski, Gregory D., Modjarrad, Kayvon, Dooley, Helen, Joyce, M. Gordon
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03.02.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 101/47; 13/1; 60 APPLIED LIFE SCIENCES; 631/154/51/1568; 631/250/255/2514; 631/535/1266; 631/61/24; 64/110; 82/80; 9/10; Affinity; Animals; Antibodies; Antibodies, Neutralizing; Antibodies, Viral; antibody therapy; Antigens; applied immunology; Chimeras; COVID-19; COVID-19 - prevention & control; Epitopes; Ferritin; Ferritins - genetics; Humanities and Social Sciences; Immunization; Immunoglobulin Fc Fragments; Mice; Mice, Transgenic; multidisciplinary; Nanobodies; Nanoparticles; Neutralization; Pandemics; Receptors; SARS-CoV-2 - genetics; Science; Science (multidisciplinary); Severe acute respiratory syndrome coronavirus 2; Severe acute respiratory syndrome-related coronavirus; Sharks; Single-Domain Antibodies; Spike Glycoprotein, Coronavirus - genetics; Spike protein; Transgenic mice; Vaccine efficacy; Vaccines; Viral diseases; viral infection; x-ray crystallography

Despite rapid and ongoing vaccine and therapeutic development, SARS-CoV-2 continues to evolve and evade, presenting a need for next-generation diverse therapeutic modalities. Here we show that nurse sharks immunized with SARS-CoV-2 recombinant receptor binding domain (RBD), RBD-ferritin (RFN), or sp...