Development of spirulina for the manufacture and oral delivery of protein therapeutics

• Published in: Nature biotechnology Vol. 40; no. 6; pp. 956 - 964
• Main Authors: Jester, Benjamin W., Zhao, Hui, Gewe, Mesfin, Adame, Thomas, Perruzza, Lisa, Bolick, David T., Agosti, Jan, Khuong, Nhi, Kuestner, Rolf, Gamble, Caitlin, Cruickshank, Kendra, Ferrara, Jeremy, Lim, Rachelle, Paddock, Troy, Brady, Colin, Ertel, Stacey, Zhang, Miaohua, Pollock, Alex, Lee, Jamie, Xiong, Jian, Tasch, Michael, Saveria, Tracy, Doughty, David, Marshall, Jacob, Carrieri, Damian, Goetsch, Lauren, Dang, Jason, Sanjaya, Nathaniel, Fletcher, David, Martinez, Anissa, Kadis, Bryce, Sigmar, Kristjan, Afreen, Esha, Nguyen, Tammy, Randolph, Amanda, Taber, Alexandria, Krzeszowski, Ashley, Robinett, Brittney, Volkin, David B., Grassi, Fabio, Guerrant, Richard, Takeuchi, Ryo, Finrow, Brian, Behnke, Craig, Roberts, James
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.06.2022; Nature Publishing Group; Springer Nature
• Subjects: 631/1647/1511; 631/61/185; 631/61/2297; 631/61/51/2314; Agriculture; Antibodies; BASIC BIOLOGICAL SCIENCES; Bioinformatics; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Biotechnology & Applied Microbiology; Campylobacter; Chromosomes; Cyanobacteria; Food safety; Genetic engineering; Infant mortality; Life Sciences; Microorganisms; Photosynthesis; Proteins; Refrigeration; Safety; Spirulina

The use of the edible photosynthetic cyanobacterium Arthrospira platensis (spirulina) as a biomanufacturing platform has been limited by a lack of genetic tools. Here we report genetic engineering methods for stable, high-level expression of bioactive proteins in spirulina, including large-scale, in...