Pseudoknot length modulates the folding, conformational dynamics, and robustness of Xrn1 resistance of flaviviral xrRNAs

• Published in: Nature communications Vol. 12; no. 1; pp. 6417 - 14
• Main Authors: Niu, Xiaolin, Sun, Ruirui, Chen, Zhifeng, Yao, Yirong, Zuo, Xiaobing, Chen, Chunlai, Fang, Xianyang
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.11.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 45/70; 45/71; 45/77; 631/45/500; 631/535/1261; 631/57/2265; 82/80; 82/83; 9/97; 96/33; BASIC BIOLOGICAL SCIENCES; Biomedical materials; Dengue fever; Dynamics; Flavivirus - genetics; Folding; Humanities and Social Sciences; Laboratories; Life sciences; Magnesium; multidisciplinary; Mutation; Nucleic Acid Conformation; Regulatory mechanisms (biology); Ribonucleic acid; RNA; RNA Folding - genetics; RNA Folding - physiology; Robustness; Science; Science (multidisciplinary); Viruses; West Nile virus

To understand how RNA dynamics is regulated and connected to its function, we investigate the folding, conformational dynamics and robustness of Xrn1 resistance of a set of flaviviral xrRNAs using SAXS, smFRET and in vitro enzymatic assays. Flaviviral xrRNAs form discrete ring-like 3D structures, in...