Structures of the ISWI–nucleosome complex reveal a conserved mechanism of chromatin remodeling

• Published in: Nature structural & molecular biology Vol. 26; no. 4; pp. 258 - 266
• Main Authors: Yan, Lijuan, Wu, Hao, Li, Xuemei, Gao, Ning, Chen, Zhucheng
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.04.2019; Nature Publishing Group
• Subjects: 631/337/100/102; 631/535/1258/1259; Adenosine; Adenosine diphosphate; Adenosine Diphosphate - metabolism; Adenosine Triphosphatases - metabolism; Adenosine Triphosphatases - ultrastructure; ATPases; Baking yeast; Biochemistry; Biological Microscopy; Biomedical and Life Sciences; Catalysis; Chromatin; Chromatin Assembly and Disassembly - genetics; Chromatin Assembly and Disassembly - physiology; Chromatin remodeling; Crosslinking; Cryoelectron Microscopy - methods; Deformation mechanisms; Deoxyribonucleic acid; Distortion; DNA; DNA structure; DNA-Binding Proteins - metabolism; DNA-Binding Proteins - ultrastructure; Domains; Electron microscopy; Enzymes; Fluorides; Histones; Histones - metabolism; Homology; Humans; Life Sciences; Membrane Biology; Microscopy; Nucleosomes - metabolism; Nucleosomes - ultrastructure; Polymer crosslinking; Protein Structure; Proteins; RecA protein; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae - ultrastructure; Saccharomyces cerevisiae Proteins - metabolism; Saccharomyces cerevisiae Proteins - ultrastructure; Transcription Factors - metabolism; Translocation; Yeast

Chromatin remodelers are diverse enzymes, and different models have been proposed to explain how these proteins work. Here we report the 3.3 Å-resolution cryogenic electron microscopy (cryo-EM) structures of Saccharomyces cerevisiae ISWI (ISW1) in complex with the nucleosome in adenosine diphosphate...