Plasma DNA tissue mapping by genome-wide methylation sequencing for noninvasive prenatal, cancer, and transplantation assessments

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 40; pp. E5503 - E5512
• Main Authors: Sun, Kun, Jiang, Peiyong, Chan, K. C. Allen, Wong, John, Cheng, Yvonne K. Y., Liang, Raymond H. S., Chan, Wai-kong, Ma, Edmond S. K., Chan, Stephen L., Cheng, Suk Hang, Chan, Rebecca W. Y., Tong, Yu K., Ng, Simon S. M., Wong, Raymond S. M., Hui, David S. C., Leung, Tse Ngong, Leung, Tak Y., Lai, Paul B. S., Chiu, Rossa W. K., Lo, Yuk Ming Dennis
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 06.10.2015; National Acad Sciences
• Series: PNAS Plus
• Subjects: Adult; Algorithms; B-Lymphocytes - metabolism; Biological Sciences; Biomarkers; Bone marrow; Bone Marrow Transplantation; Carcinoma, Hepatocellular - blood; Carcinoma, Hepatocellular - genetics; Deoxyribonucleic acid; DNA; DNA - blood; DNA - chemistry; DNA - genetics; DNA Copy Number Variations - genetics; DNA Methylation; Female; Fetus - metabolism; Genetic markers; Genomes; Humans; Liver - metabolism; Liver - pathology; Liver Neoplasms - blood; Liver Neoplasms - genetics; Liver Transplantation; Lymphoma; Methylation; Middle Aged; Neutrophils - metabolism; Placenta - metabolism; Plasma; PNAS Plus; Pregnancy; Sequence Analysis, DNA - methods; T-Lymphocytes - metabolism; Tissue Transplantation; Tissues; Transplants & implants; transplantation monitoring; noninvasive prenatal testing; epigenetics; liquid biopsy; circulating tumor DNA

Plasma consists of DNA released from multiple tissues within the body. Using genome-wide bisulfite sequencing of plasma DNA and deconvolution of the sequencing data with reference to methylation profiles of different tissues, we developed a general approach for studying the major tissue contributors...