Analytical methods involving separation techniques for determination of low-molecular-weight biothiols in human plasma and blood

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 964; pp. 103 - 115
• Main Authors: Isokawa, Muneki, Kanamori, Takahiro, Funatsu, Takashi, Tsunoda, Makoto
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2014
• Subjects: antioxidant activity; biomarkers; Blood; blood plasma; capillary electrophoresis; cardiovascular diseases; Chemiluminescence; Chromatography, High Pressure Liquid - methods; Cysteine; Cysteine - blood; Cysteine - isolation & purification; Cysteine - urine; Derivatization; Diseases; Electrophoresis; Electrophoresis, Capillary - methods; Fluorescence; Glutathione; Glutathione - blood; Glutathione - isolation & purification; Glutathione - urine; high performance liquid chromatography; homocysteine; Homocysteine - blood; Homocysteine - isolation & purification; Homocysteine - urine; HPLC; Human; Humans; Luminescent Measurements - methods; mass spectrometry; Mass Spectrometry - methods; Mathematical analysis; Medical services; metabolites; methionine; neoplasms; neurodegenerative diseases; protein synthesis; Spectrometry, Fluorescence - methods; Spectrophotometry, Ultraviolet - methods; Sulfhydryl Compounds - blood; Sulfhydryl Compounds - isolation & purification; Sulfhydryl Compounds - urine; sulfur; thiols; Fluorescence; Chemiluminescence; Derivatization; HPLC

•Biothiols play important roles in biological processes.•Thiol concentrations in human plasma and blood are related to diseases.•We review analytical methods for biothiols applied to human plasma and blood. Low-molecular-weight biothiols such as homocysteine, cysteine, and glutathione are metabolite...