High-throughput mapping of RNA solvent accessibility at the single-nucleotide resolution by RtcB ligation between a fixed 5′-OH-end linker and unique 3′-P-end fragments from hydroxyl radical cleavage

• Published in: RNA biology Vol. 19; no. 1; pp. 1179 - 1189
• Main Authors: Solayman, Md, Litfin, Thomas, Zhou, Yaoqi, Zhan, Jian
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 31.12.2022; Taylor & Francis Group
• Subjects: Amino Acyl-tRNA Synthetases - genetics; Escherichia coli; Escherichia coli - genetics; Escherichia coli Proteins - genetics; High-Throughput Nucleotide Sequencing - methods; hydroxyl radical; Hydroxyl Radical - chemistry; hydroxyl radicals; Nucleic Acid Conformation; Nucleotides; probing; Research Paper; reverse transcription; ribosomes; RL-Seq; RNA; RNA - chemistry; RNA - genetics; RNA-directed DNA polymerase; SASA; solvent accessibility; solvents; Solvents - chemistry; probing; hydroxyl radical; SASA; RL-Seq; solvent accessibility

Given the challenges for the experimental determination of RNA tertiary structures, probing solvent accessibility has become increasingly important to gain functional insights. Among various chemical probes developed, backbone-cleaving hydroxyl radical is the only one that can provide unbiased detec...