Overcoming the thermodynamic equilibrium of an isomerization reaction through oxidoreductive reactions for biotransformation

• Published in: Nature communications Vol. 10; no. 1; pp. 1356 - 8
• Main Authors: Liu, Jing-Jing, Zhang, Guo-Chang, Kwak, Suryang, Oh, Eun Joong, Yun, Eun Ju, Chomvong, Kulika, Cate, Jamie H. D., Jin, Yong-Su
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.03.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 38/22; 38/44; 38/77; 45/41; 631/1647/1511; 631/326/2522; 631/61/318; 82/80; Aldehyde Reductase - metabolism; BASIC BIOLOGICAL SCIENCES; Bioreactors - microbiology; Biotransformation; Cofactors; Galactose; Galactose - metabolism; Gene Dosage; Hexoses - metabolism; Humanities and Social Sciences; Intracellular Space - metabolism; Isomerism; Isomerization; Lactose; Lactose - metabolism; Models, Biological; multidisciplinary; Oxidation-Reduction; Oxidoreductase; Oxidoreductases - metabolism; Saccharomyces cerevisiae - metabolism; Science; Science (multidisciplinary); Substrates; Sugar Alcohol Dehydrogenases - metabolism; Thermodynamic equilibrium; Thermodynamics; Xylose; Xylose - metabolism; Xylose reductase; Yeast

Isomerases perform biotransformations without cofactors but often cause an undesirable mixture of substrate and product due to unfavorable thermodynamic equilibria. We demonstrate the feasibility of using an engineered yeast strain harboring oxidoreductase reactions to overcome the thermodynamic lim...