超高效液相色谱-串联质谱法测定鸡皮及脂肪组织中抗球虫类药物残留

建立了检测鸡的皮和脂肪中抗球虫类药物的超高效液相色谱-串联质谱分析方法.样品采用甲醇、乙腈、乙酸（4∶ 1∶0.05; V/V）混合溶液提取,提取液经Sep-Pak tC18固相萃取柱富集净化,在电喷雾正离子模式下,以多反应监测模式采集数据,进行定性与定量分析.结果表明,常山酮和氯苯胍的检出限均为7.0 μg/kg,定量限为20μg/kg;盐霉素、莫能菌素、甲基盐霉素、马杜霉素和拉沙洛菌素的检出限均为5.0 μg/kg,定量限为15.0 μg/kg.本方法在15 ～ 200 μg/kg添加浓度范围内的回收率为75％～110％,日内相对标准偏差≤12.8％,日间相对标准偏差≤13.4％....

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Bibliographic Details
Published in分析化学 Vol. 42; no. 3; pp. 409 - 414
Main Author 王盈予 夏曦 李晓薇 丁双阳 沈建忠
Format Journal Article
LanguageChinese
Published 中国农业大学动物医学院,北京,100193 2014
Subjects
串联质谱
抗球虫药
残留
超高效液相色谱
Ultra performance liquid chromatography
Tandem mass spectrometry
Coccidiostats
残留
超高效液相色谱
串联质谱
抗球虫药
Residue
Online AccessGet full text
ISSN0253-3820
DOI10.3724/SP.J.1096.2014.30756

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Summary:建立了检测鸡的皮和脂肪中抗球虫类药物的超高效液相色谱-串联质谱分析方法.样品采用甲醇、乙腈、乙酸（4∶ 1∶0.05; V/V）混合溶液提取,提取液经Sep-Pak tC18固相萃取柱富集净化,在电喷雾正离子模式下,以多反应监测模式采集数据,进行定性与定量分析.结果表明,常山酮和氯苯胍的检出限均为7.0 μg/kg,定量限为20μg/kg;盐霉素、莫能菌素、甲基盐霉素、马杜霉素和拉沙洛菌素的检出限均为5.0 μg/kg,定量限为15.0 μg/kg.本方法在15 ～ 200 μg/kg添加浓度范围内的回收率为75％～110％,日内相对标准偏差≤12.8％,日间相对标准偏差≤13.4％.
Bibliography:Ultra performance liquid chromatography; Tandem mass spectrometry; Coccidiostats; Residue
22-1125/O6
WANG Ying-Yu, XIA Xi, LI Xiao-Wei, DING Shuang-Yang, SHEN Jian-Zhong ~ （College of Veterinary Medicine, China Agricultural University, Beijing 100193, China）
A method based on ultra performance liquid chromatography-tandem mass spectrometry （UPLC- MS/MS） has been proposed for the determination of coccidiostat residues in chicken skin and fat. The sample was extracted with the combination of methanol, acetonitrile, and acetic acid, and cleaned-up by Sep-Pak tC18 solid phase extraction cartridge. Data acquisition under positive electrospray mode was performed by applying multiple reaction monitoring for both identification and quantification. The limits of detection and quantification for halofuginone and robenidine were 7 μg/kg and 20 μg/kg, respectively. The limit of detection of salinomycin, monensin, narasin, maduramicin, and lasalocid was 5 μg/kg, and limit of quantification was 15 p,g/kg. The recovery was 75%
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2014.30756